Thursday 18 November 2021

What are the different types of gel electrophoresis available?

 

Whenever you are researching about proteins and nucleic acid, there is a great possibility that you will be running your samples through a gel electrophoresis. This is considered to be one of the most efficient techniques to separate your proteins and DNA from a mixture. There are two types of gel electrophoresis available for research. They are horizontal gel electrophoresis and vertical gel electrophoresis. The basic differences between the two are as follows:

In horizontal gel electrophoresis the technique used in which the gel is present is horizontally oriented. Whereas in vertical gel electrophoresis, the gel technique is present in a vertical orientation.

Horizontal gel electrophoresis has continuous buffer where as in the vertical one, the running buffer is discontinuous in nature

The use of Acrylamide is not possible for horizontal since the gel box is exposed to atmospheric oxygen. In the case of vertical one, there are two chambers used and the atmospheric oxygen is not exposed.

Horizontal one is mostly used for the separation of RNA and DNA mixture whereas the vertical one is used for separating of proteins from the mixture.

If you are looking for any of the gel electrophoresis, iGene Labserve can be great company. They offer the best of the products used for research and other life science projects. They are one of the most reputed names in the industry with years of experience in the business. For more information, log on to www.igenels.com

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