The polymerase chain reaction (PCR) machine is used to make millions even billions of copies of a single piece of DNA. It is an extremely crucial tool used for the science technology which has transformed the study of research and diagnostic medicine. It helps in identifying many genes related to phenotypes including genetic disorders. PCR machine helps in focusing on the actual segment of DNA that is of interest rather than looking at the entire genome. With the help of this machine small segment of genotypes can be determined and as a result many genetic disorders can be detected and monitored.
Many pathogenic microorganisms such as parasites, bacteria, viruses and fungi can be detected with the help of this machine. PCR is also very effective molecular diagnostics and biochemical analyses. These techniques can be used in the process of drug development, specifically in measuring the efficacy of drug therapy and research into Cancer detection and treatment.
The amplified DNA produced by this machine has a number of uses which includes the investigation of genetic diseases, DNA fingerprinting and detection of parasites, bacteria and viruses. This is the reason, PCR is used in biomedical research and criminal forensics.
Another important system used for research and development is vertical gel electrophoresis system. This system is used to separate proteins instead of nucleic acid. Before separating the protein it needs to be broken down into linear strands. After the protein is broken into linear strands, the vertical gel electrophoresis system is used to separate the strands. This gel uses polyacrylamide gel which has smaller pore size. The linear protein strands are smaller than DNA and RNA. Thus a smaller pore size of polyacrylamide gel is used to separate them.
The user pours the gel solution between a set of glass plates to form a thin gel. The buffer flows through the gel in the vertical system. When combined with polyacrylamide gel, it offers better separation and resolution. This system uses two layers gel which are stacking gel and resolving gel. It is relatively more difficult to set up and run under denaturing conditions. In this system, the sample size must be able to run through the pore size to get optimum results. Choosing the right buffer is also important for your experiment. It’s a unique and leak proof design.
Biosafety cabinets are used in the laboratory to offer personnel, environmental and product protection. It is very effective in preventing exposure to biohazards. There are many designs available in these cabinets. They can be classified as Class I, Class II and Class III in the United States. Class I type of cabinets provide personnel and environment protection, whereas Class II and Class III cabinets provide personnel, environmental and product protection. Class II cabinets are widely used in the United States and the types available are A1, A2, B1 and B2.
These types of cabinets are only used for certain risk group organisms and for processes that might result in aerosol formation. These cabinets are built with HEPA filters that clean out the air moving out of the cabinet. They minimize the formation of aerosol and protects the personnel, environment and the pathogens. Other than this, these cabinets are also used to sterilize the biological materials kept inside the cabinet.
If you are looking for PCR machine, vertical gel electrophoresis system, and biosafety cabinet, you can look at iGene Labserve. They are one of the finest in the industry and have made a mark in the market with their quality products. They have list of satisfied customers who keep coming back to them. For more information, log on to www.igenels.com.
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