Ultra-low temperature freezers (ULT) are used by laboratories for storing drugs, enzymes, biological materials, tissue samples etc. Maintaining a temperature between -30° to -40° Fahrenheit, the low temperature is ideal for storing sensitive materials that have high perishability.
The polymerase chain reaction (PCR) machine is used to make millions even billions of copies of a single piece of DNA. It is an extremely crucial tool used for the science technology which has transformed the study of research and diagnostic medicine. It helps in identifying many genes related to phenotypes including genetic disorders. PCR machine helps in focusing on the actual segment of DNA that is of interest rather than looking at the entire genome. With the help of this machine small segment of genotypes can be determined and as a result many genetic disorders can be detected and monitored.
Many pathogenic microorganisms such as parasites, bacteria, viruses and fungi can be detected with the help of this machine. PCR is also very effective molecular diagnostics and biochemical analyses. These techniques can be used in the process of drug development, specifically in measuring the efficacy of drug therapy and research into Cancer detection and treatment.
The amplified DNA produced by this machine has a number of uses which includes the investigation of genetic diseases, DNA fingerprinting and detection of parasites, bacteria and viruses. This is the reason, PCR is used in biomedical research and criminal forensics.
Another important system used for research and development is vertical gel electrophoresis system. This system is used to separate proteins instead of nucleic acid. Before separating the protein it needs to be broken down into linear strands. After the protein is broken into linear strands, the vertical gel electrophoresis system is used to separate the strands. This gel uses polyacrylamide gel which has smaller pore size. The linear protein strands are smaller than DNA and RNA. Thus a smaller pore size of polyacrylamide gel is used to separate them.
The user pours the gel solution between a set of glass plates to form a thin gel. The buffer flows through the gel in the vertical system. When combined with polyacrylamide gel, it offers better separation and resolution. This system uses two layers gel which are stacking gel and resolving gel. It is relatively more difficult to set up and run under denaturing conditions. In this system, the sample size must be able to run through the pore size to get optimum results. Choosing the right buffer is also important for your experiment. It’s a unique and leak proof design.
Biosafety cabinets are used in the laboratory to offer personnel, environmental and product protection. It is very effective in preventing exposure to biohazards. There are many designs available in these cabinets. They can be classified as Class I, Class II and Class III in the United States. Class I type of cabinets provide personnel and environment protection, whereas Class II and Class III cabinets provide personnel, environmental and product protection. Class II cabinets are widely used in the United States and the types available are A1, A2, B1 and B2.
These types of cabinets are only used for certain risk group organisms and for processes that might result in aerosol formation. These cabinets are built with HEPA filters that clean out the air moving out of the cabinet. They minimize the formation of aerosol and protects the personnel, environment and the pathogens. Other than this, these cabinets are also used to sterilize the biological materials kept inside the cabinet.
If you are looking for PCR machine, vertical gel electrophoresis system, and biosafety cabinet, you can look at iGene Labserve. They are one of the finest in the industry and have made a mark in the market with their quality products. They have list of satisfied customers who keep coming back to them. For more information, log on to www.igenels.com.
Whenever you are researching about proteins and nucleic acid, there is a great possibility that you will be running your samples through a gel electrophoresis. This is considered to be one of the most efficient techniques to separate your proteins and DNA from a mixture. There are two types of gel electrophoresis available for research. They are horizontal gel electrophoresis and vertical gel electrophoresis. The basic differences between the two are as follows:
In horizontal gel electrophoresis the technique used in which the gel is present is horizontally oriented. Whereas in vertical gel electrophoresis, the gel technique is present in a vertical orientation.
Horizontal gel electrophoresis has continuous buffer where as in the vertical one, the running buffer is discontinuous in nature
The use of Acrylamide is not possible for horizontal since the gel box is exposed to atmospheric oxygen. In the case of vertical one, there are two chambers used and the atmospheric oxygen is not exposed.
Horizontal one is mostly used for the separation of RNA and DNA mixture whereas the vertical one is used for separating of proteins from the mixture.
If you are looking for any of the gel electrophoresis, iGene Labserve can be great company. They offer the best of the products used for research and other life science projects. They are one of the most reputed names in the industry with years of experience in the business. For more information, log on to www.igenels.com
Sample contamination can be disastrous. It means the loss of important material and precious time. There is a solution for it, Biosafety cabinets. If you are looking for contaminant free environment Biosafety cabinets are the best bet. They are used to protect the environment and the users from pathogens. Biosafety cabinet offer an embedded and ventilated workplace. The embedded space improves safety when working with contaminated by pathogens. These cabinets are utilized where a defined Biosafety is needed. All cabinets go through meticulous testing before they are made to use. These cabinets are deemed to be very useful in reducing laboratory induced infections and cross contamination. These cabinets need to be certified periodically to improve their efficiency. Different kinds of field tests are performed to check the air flow, HEPA filter and check of contamination of the air. These cabinets work best for processes that require sterile work zone.
There are different types of Biosafety cabinets available in the market such as Class I, Class II and Class II. They provide different level of protection to the user and the research material. In Class I type of cabinets, you will get personnel and environmental protection but no product protection. These cabinets are used for low to moderate risk biological pathogens. Whereas the Class II and III of Biosafety cabinets ensure environmental, personnel as well as product protection.
The main purpose of these cabinets is to protect the users and the environment from pathogens. There is a tendency of formation of aerosols during the process of microorganisms.
If there is a certain risk involved then only the Biosafety cabinet are used since aerosols might be formed in the process.
These cabinets are facilitated with HEPA filters that help in decontaminating the air moving out of the cabinet.
Laminar hoods are also available which might be confused with these cabinets as they both are used as enclosed work places. But Laminar hoods protect only the samples where as the cabinets protect the environment, the personnel and the samples.
Most of these cabinets also help in sterilizing the biological materials that are kept inside the cabinet.
Know your airflow: If you know the functioning of filtered and contaminated air, it would be easier to maintain and get the maximum potential of these cabinets
Never block the airflow grill: Avoid covering the air grill. It performs the job of separating the clean air inside from the contaminated air outside the cabinet
Minimize movement: Ensure that you take your hand out perpendicularly while working in your cabinets. This helps in minimizing the disruption of airflow within the cabinet.
If you are searching for Biosafety cabinets, you have come to the right place. We at I Gene Labserve offer the finest quality of cabinets to help you in your laboratory needs. We have different types to select from. For more information, log on to www. Igenels.com.
A shaking water bath is used for mixing and agitating samples in a water bath under controlled temperature. Compared to other types of ...
