Understanding the basics of stability chamber

 

Pharmaceutical, food technology, biomedical storage, life science, and other industries employ stability chambers in research and development. Stability chambers can even be used to detect a flaw in a prototype product before it is put into full-scale manufacturing.

Chambers exist in a variety of sizes, and some are simple while others can execute a wide range of tasks. Any company considering purchasing a stability chamber should think about their needs as well as the quantity of space available in their facility for such equipment.

Humidity capsules are used to adjust the humidity levels within a chamber. Prior to testing, these capsules are perforated and placed in the chamber. The test substance is subsequently placed in the test compartment, which is then sealed with a specific sensor cover. This cap captures and sends critical information that aids in the research of test materials.

Stability Testing Chambers

Chemical, biological, and microbiological studies can be conducted and monitored in a Stability Testing Chamber. Products can be evaluated in the chamber to see how elements like humidity, light, and temperature affect them while they're being stored. Companies can determine the length of time a product can be stored under specific conditions and the shelf-life of the drug by testing the factors that cause changes in the quality of the drug. Pharmaceutical companies can also assess how environmental changes affect the quality of a product by closely monitoring it in testing chambers.

Stability Applications Include:

• accelerated aging
• shelf life testing
• stability testing
• expiration date testing

How do I choose a laboratory stability chamber?

Because there are so many different types and sizes of test chambers/stability chamber to choose from, it's critical to do your homework before you buy. Take the time to list the features that are required for your test procedure or storage requirements:

• Chambers can reach far over 200 °C for high-temperature applications, depending on the material used in their construction.
• CO2, which can drop a chamber's temperature to –73 °C relatively rapidly, and LN2, which can drop it to –185 °C, are two refrigeration possibilities. A single-stage compressor or a two-stage cascade compressor can be used for mechanical refrigeration.
• Keep in mind the unit's pull-down and ramp-up times, as they are influenced by a number of factors, including the unit's size, temperature range, and heating or refrigeration system's power.
• Although the most typical relative humidity range is 20–98% RH, dry air purge devices can reduce this even more.
• Microprocessor-based controllers, autotune, LED, LCD, or touch LCD temperature and RH displays, and solid-state RH sensors are all features to look for.
• There are benchtop, reach-in, and walk-in variants to choose from. Leaks are less likely with seam welding of the interior chamber walls and a correctly fitting door gasket.

iGene Labserve is an Indian company that manufactures professional scientific equipment. Our organisation primarily concentrates on the production of laboratory instruments for a variety of scientific research fields, including Molecular Biology, Analytical Chemistry, Microbial Technology, and other life science-related research labs.

What does a PCR machine do?

The thermocycler, or polymerase chain reaction-PCR machine, is a low-cost and high-efficiency technique for amplifying short portions of DNA or RNA. PCR uses the concepts of nucleic acid replication and complementary nucleic acid hybridization to create specific target DNA/RNA sequences exponentially by a factor of 107 in a couple of hours.

The polymerase chain reaction process can be classified into three stages:

1. Denaturing – In this first process the DNA is heated into 2 separate pieces of single-strand DNA at around 94-95°C.
2. Annealing – In the second process, the temperature is lowered, which enables the primers to bind DNA and initiate the polymerization at 50-56°C.
3. Extending – The temperature is raised again in the third process by using the original strands as templates. New strands of DNA are made by an enzyme at 72°C.

One cycle of PCR results in two strands of DNA. Each of these strands contains one original strand and one newly made strand.

The three strands of DNA make up one full cycle and the cycle is repeated around 30 times. After which each one doubles the number of DNA copies, which allows the scientists to create millions of copies needed to research a single piece of DNA.

The cycling process of PCR is automatic and can create billion of copies within 2-3 hours. It gets challenging to come across a quality PCR Machine, for which we would like to suggest Igene. It is a reliable scientific equipment manufacturer to help you find the best laboratory Instrumentation for Healthcare and more. Contact https://www.igenels.com or dial 09310696848 to know more about the machine.

The Uses of Laminar Airflow Benches

What is a laminar airflow cabinet?

A laminar airflow cabinet is a closed cabinet fitted with HEPA (High-Efficiency Particulate Air) filter airflow system. The air flows with a steady, uniform velocity of 0.3 m/s to 0.5 m/s. The inwards flow of air through one or more HEPA filters creates a particulate-free environment for carrying out specialised work. The instrument protects the product from contaminants. Laminar airflow hoods work with biological samples, sensitive materials, and semiconductors. The Laminar flow hood has undergone a massive change since it was first developed in the 1960s. Keeping the air safety in check, in laboratory enclosure, this product is still quite relevant in all scientific research. The performance varies with the work performed and the principle is guided by unidirectional airflow and maintenance of sterility preventing cross-contamination and reducing turbulence. The efficiency of this filter in removing 99.9998% of all particles >0.12 μm in size and streamlining the airflow both vertically and horizontally across the work surface.

Where can laminar airflow be used?

Medical labs: Sterile environment is required in labs to prevent airborne contaminants affecting samples in experiments. The UV lamp sterilizes the workplace and inhibits contaminants such as bacteria and viruses from travelling. When working on the laminar airflow, scientists and researchers switch off the UV light to protect the operator from exposure.

Tissue Culture: For bio-scientists, tissue culture is an integral part of cell study in a multicellular organism. Tissues are manipulated and analysed in a well-defined atmosphere. The contamination-free environment makes it ideal for cultures to be tested and analysed. 

Food processing: Useful in the packaging of small-scale food, laminar airflow cabinets are used in packaging and processing by food formulation and testing labs.

Pharmaceutical production: Before a drug enters the market, enormous research is undertaken by scientists to develop it. For the process of formulating the drug, scientists need to take help from the laminar airflow benches where they do sample study and carry out experiments.

Medical equipment manufacture: When medical equipment is manufactured it is important to maintain sterility for ensuring that the equipment is free from contamination. Laminar airflow is used during the manufacturing process.

Assembling Electronic parts: The delicate microchip is extremely sensitive to atmospheric contamination. A laminar airflow provides the right resistance from any form of contagion. These chips are tested and assembled in the laminar airflow cabinet.

Protection to movie lenses: Dust that easily attaches to lenses creates marks on the film when in use and can spoil the film quality. Modern movie projections are assembled in laminar airflow technology to keep dust away from modern movie projections.

Data Rescue: Laminar airflow benches are used for data retrieval from hard drives, flash drives and other digital devices.

What Precautions Must you take before using the Laminar airflow benches?

• Considering the following while using the Laminar airflow benches will keep the system and the user safe. 
• Sterilize the Laminar airflow benches with UV light pre-and-post-operation. 
• Remember to switch off the UV light and airflow when working on the Laminar airflow benches.
• Wear lab coat and long gloves during operation. 
• Sterilize thoroughly the working bench, glass shield, and other components present inside the cabinet after work is over.
  

For the safest and smoothest operation, you can choose iGeneLabservePvt. Ltd. They are an emerging and enterprising laboratory instruments maker. Their care and safety measures along with accuracy in instruments have already earned them a good reputation in the market. To contact them visit. https://www.igenels.com/.