Environment, Health & Safety - Biosafety Cabinet

 

A vented enclosure known as a biological safety cabinet protects people handling potentially dangerous microorganisms from aerosols that might harm them, their products, or the environment. This post will talk about the use of a HEPA filter, the constant airflow is released into the atmosphere and the use of biosafety cabinet.

Different Biosafety Levels

Biosafety Level 1 pertains to the use of biological agents, which provide a small danger or harm to lab workers and the environment. Without using specialized containment apparatus, work with these kinds of chemicals is often carried out in open laboratory cabinets.
 

Biosafety Level 2 includes handling harmful or infectious organisms that pose a medium risk. Salmonella, the Hepatitis B virus, and the measles virus are a few examples.

Biosafety Level 3 apply when dealing with domestic or foreign agents that have the potential to spread deadly or severe illness through aerosols.

In Biosafety Level 4, apply when handling substances that are exceedingly lethal, infectious, and life-threatening. Ebola, the Lassa virus, and any sample with unidentified risks of pathogenicity and transmission are examples.

Let’s understand about 3 different class of Biosafety cabinet

Class 1 Biological Safety Cabinets

When working with chemicals and powders, the Class 1 biological safety cabinet offers both environmental and people protection. A built-in exhaust fan, HEPA filter, and/or carbon filter are used to safeguard the environment and the operator when the air reaches the cabinet through the top aperture. Following that, the air leaves the cabinet at the back of the work surface. Therefore, the front aperture's inward inflow and the filtration/absorption of the exhaust air serve to regulate the escape of any airborne particles formed inside the cabinet.

Class 2 Biological Safety Cabinets

The standards for human, environmental, and product protection must be met by the Class 2 biological safety cabinet. In the Biosafety Cabinet Class II B2 ,the aperture sucks in air into the work chamber. It then travels underneath the work surface and up the rear plenum, where 70% of it is recirculated through the primary HEPA filter to produce down flow and 30% of it is expelled. The vertical laminar flow biological safety cabinet protects the operator through the input, the product through the down flow, and the environment through the filtered exhaust.

Class 3 Biological Safety Cabinets

The operator is physically isolated from their work by a Class 3 Biological Safety Cabinet, which is an aerosol-tight container with a non-opening, totally sealed front glass. The cabinet is often referred to as a "Glovebox". Long, sturdy rubber gloves that are gas-tightly fastened to the apertures on the front of the safety cabinet are used for work. This permits access to and work on your goods within the cabinet without jeopardizing containment.

A safety airlock transfer hatch or double door autoclave, which is situated on the side of the chamber of the BSC for decontamination, must be used to remove materials from the cabinet. To prevent both doors from being open at once, procedures or interlock must be employed on the autoclave and pass-through doors. Visit IGene Labserve at https://www.igenels.com or call 09310696848 to speak with someone and order your very own biohazard safety cabinets.