Environment, Health & Safety - Biosafety Cabinet

 

A vented enclosure known as a biological safety cabinet protects people handling potentially dangerous microorganisms from aerosols that might harm them, their products, or the environment. This post will talk about the use of a HEPA filter, the constant airflow is released into the atmosphere and the use of biosafety cabinet.

Different Biosafety Levels

Biosafety Level 1 pertains to the use of biological agents, which provide a small danger or harm to lab workers and the environment. Without using specialized containment apparatus, work with these kinds of chemicals is often carried out in open laboratory cabinets.
 

Biosafety Level 2 includes handling harmful or infectious organisms that pose a medium risk. Salmonella, the Hepatitis B virus, and the measles virus are a few examples.

Biosafety Level 3 apply when dealing with domestic or foreign agents that have the potential to spread deadly or severe illness through aerosols.

In Biosafety Level 4, apply when handling substances that are exceedingly lethal, infectious, and life-threatening. Ebola, the Lassa virus, and any sample with unidentified risks of pathogenicity and transmission are examples.

Let’s understand about 3 different class of Biosafety cabinet

Class 1 Biological Safety Cabinets

When working with chemicals and powders, the Class 1 biological safety cabinet offers both environmental and people protection. A built-in exhaust fan, HEPA filter, and/or carbon filter are used to safeguard the environment and the operator when the air reaches the cabinet through the top aperture. Following that, the air leaves the cabinet at the back of the work surface. Therefore, the front aperture's inward inflow and the filtration/absorption of the exhaust air serve to regulate the escape of any airborne particles formed inside the cabinet.

Class 2 Biological Safety Cabinets

The standards for human, environmental, and product protection must be met by the Class 2 biological safety cabinet. In the Biosafety Cabinet Class II B2 ,the aperture sucks in air into the work chamber. It then travels underneath the work surface and up the rear plenum, where 70% of it is recirculated through the primary HEPA filter to produce down flow and 30% of it is expelled. The vertical laminar flow biological safety cabinet protects the operator through the input, the product through the down flow, and the environment through the filtered exhaust.

Class 3 Biological Safety Cabinets

The operator is physically isolated from their work by a Class 3 Biological Safety Cabinet, which is an aerosol-tight container with a non-opening, totally sealed front glass. The cabinet is often referred to as a "Glovebox". Long, sturdy rubber gloves that are gas-tightly fastened to the apertures on the front of the safety cabinet are used for work. This permits access to and work on your goods within the cabinet without jeopardizing containment.

A safety airlock transfer hatch or double door autoclave, which is situated on the side of the chamber of the BSC for decontamination, must be used to remove materials from the cabinet. To prevent both doors from being open at once, procedures or interlock must be employed on the autoclave and pass-through doors. Visit IGene Labserve at https://www.igenels.com or call 09310696848 to speak with someone and order your very own biohazard safety cabinets.

Important Features of Gradient Thermal Cycler

 

 

Thermal gradient and Peltier effect technologies, which make it simple to readily tune PCR tests in a single run, are common aspects of Gradient thermal cyclers.

PRECISION THERMAL CONTROL: Six Peltier modules are used to quickly heat and cool the precisely cut sample block.

 GRADIENT CAPABILITY: The IGene Labserve Gradient thermal cycler block can be programmed to operate with uniform temperature across the block for consistent results, or with a temperature gradient for protocol optimization.

SLIDING, ADJUSTABLE HEATED LID: The heated lid, a standard feature of the IGene Labserve Gradient thermal cycler, is fully adjustable to provide the proper pressure for use with different height tubes as well as plates.

PROGRAMMING FEATURES: A variety of applications can be performed with the IGene Labserve Gradient thermal cycler, from single-temperature soaks to sophisticated multi step programs.

Types, Applications, and Instructions for Laboratory Fume Hood

This post will tell you all about laboratory fume hoods. We will talk about the efficiency of the Fume Hood system, and all the factors that makes this product an amazing buy. Let’s discuss some details about its construction and where you can find a quality fume hood.

Partially enclosed work rooms with exhaust vents to the exterior are called fume hoods in laboratories. They serve the main function of excluding poisonous or unpleasant gases from the main laboratory work space. When an explosive reaction could occur, a secondary function is to safeguard the specimen or act as a barrier between the user and the apparatus being utilized.

Laboratory fume hoods

They are made up of the hood and a sash, which is the front panel that can be opened and closed to increase access and decrease airflow.

Hood face velocity 

A measurement of hood face velocity is the rate of air flow across the fictitious plane connecting the bottom of the sash to the work surface. The hood face velocity is often expressed in feet per minute (fpm), and the higher it is, the faster poisons and other vapors can be removed from the system.

Fume Hood Efficiency

The needed air flow and hood face velocity of a fume hood are used to determine its efficiency.

Required airflow 

In that it measures the volume of air needed to produce laminar flow at a speed of 100 feet per minute, necessary airflow is related to hood face velocity.

Hood face velocity of 100 fpm is often used to operate general purpose fume hoods. For use with particular chemicals and technology, stronger fume hoods are available. Radioisotope and perchloric acid hoods are two popular varieties.

Radioisotope hood systems 

One of the best radioisotope hood systems are built of welded stainless steel to prevent radioactive material absorption. A face velocity of 125 fpm is necessary for radioisotope hoods in order to meet licensing standards.

 Perchloric acid

Wash-down capabilities are included into perchloric acid hoods to stop the accumulation of explosive perchlorate salts in the exhaust systems.

Construction Types

Although unique designs are available, there are five primary forms of hood structure.  These comprise:

Conventional hoods

They are the earliest and most basic hood design kind. No of the height of the sash, a typical hood always exhausts the same amount of air. (Left-side image)

Bypass Hood

To provide outside air to the face of the bypass hood, auxiliary air hoods have attached special ducts. An auxiliary air hood's principal benefit is the energy savings brought about by a reduction in the amount of heated or air-conditioned room air that is expelled by the hood.

Auxiliary air hoods 

The front of the bypass hood is supplied with outside air via auxiliary air hoods that are connected to special conduits. This hood's principal benefit is the energy savings gained by lowering the amount of heated or air-conditioned room air that the hood exhausts.

Ductless fume hoods 

In order to circulate air back into the lab after filtering, ductless fume hoods use a standard hood design. High Efficiency Particulate Air (HEPA) filters or Activated Carbon Filtration (ACF) technologies are both used by these hoods to remove impurities from the hood air.

Variable air volume (VAV) hoods 

The most complex hood types are variable air volume (VAV) hoods, which need highly skilled design, installation, and maintenance. The capacity of VAV hoods to maintain a consistent face velocity when sash height varies is its key feature.

If you're seeking to get a dependable Laboratory Fume Hood, get in touch with IGene Labserve by going to https://www.igenels.com/ or calling 09310696848 to learn everything there is to know about the item.