What Do You Know About PCR Workstation?

It might be challenging to fully comprehend the characteristics of a PCR workstation and determine whether you require one for your application because of its various applications and functions. It might be challenging to choose which PCR workstation type is appropriate for your requirements after you understand what one is. There are several different varieties available. However, don't let that scare you away; with a little background information, you'll be able to choose the best PCR workstation.

When to Use a PCR Workstation?

Although PCR workstations are frequently associated with medical applications, they have a wide range of other uses as well. They operate best in tasks that demand particle-free air and aim to safeguard the procedure being performed. Preparation of media plates, plant tissue cultures, electrical device construction, and mycology are a few examples. A PCR workstation is ideal for any application where you want to safeguard your work from contamination, at the end.

Learning what PCR workstations can and cannot be used for is just as crucial as knowing what they can. If you want to shield the operator from the substances being worked on, you shouldn't utilize them. After understanding the PCR Workstation uses, let’s get into the benefits of this equipment.

Benefits of a PCR Workstation

•   Filter replacement accuracy is ensured by a filter safety lockout.

• UV light is reflected by the architecture of polypropylene and polycarbonate.

• When the sash is lifted, the sash safety switches immediately shuts off the UV light and switches on the blower.

• When the sash is closed, an automatic sash switch shuts off the blowers to maintain the air barrier.

• UV radiation protection using overlapping polycarbonate sashes

• Tested to UL 61010-1, CSA C22.2 No. 61010-1, and EN 61326-1

• A visible and audible low airflow alarm alerts the user.

• A visible and audible low airflow alarm alerts the user.

• Real-time monitoring of a filter bed installed

• Since the filter and electronics are above the workplace, there is little danger that a chemical leak will get into these delicate regions.

These are some of the things to understand about PCR Workstation. If you have any questions about the equipment, bring down your query to IGene Labserve by visiting https://www.igenels.com/ or dial 09310696848 to understand everything about the product.

Do You Know The Important Components Of Gel Documentation System?

A gel documentation system is a system used specifically for molecular biology labs for the reason of imaging and documentation of nucleic acids along with protein that are suspended in agarose or polyacrylamide gels. The product is also called gen imager, gel doc system or gen imaging system.

Number of components used in the Gel Documentation System

Source of Irradiation

The first component is the transilluminator, it is the source of irradiation and used for observing of DNA bands. The UV lights are utilized for the radiation of these samples; however, LED light options are available as well. The lights also come with options of variable settings for its intensity. Safety cut-off values help in preventing any damage to the sample as well as the operator.

Base Plate

The base plate, which is a sample tray, is readily removable. It holds the gel so that it may be seen. It is set down on a dark, non-reflective surface.

Hood 

It serves as a UV protection darkroom for the skin. Applications involving fluorescence, chemiluminescence, and visible light can use it. To prevent prolonged radiation exposures, there is an electronic automatic door lock.

Filters

The eyes should be completely protected from UV rays with amber filters. In order to prevent noise from showing up on photographs that are being recorded, it is also used to obscure background light.

Visible Light and Adjustable Stage

The applicability of transmitted light is expanded using visible light. The screen is transformed for applications requiring visible light. It is employed to bring the sample up towards the camera.

Imaging System

It has a higher resolution camera along with the camera controller and printer. The resolution of the camera normally ranges from 1.4 MP to 8.3 MP where flatbed scanners and CCD cameras are used.

Readout System

A traditional monitor or touch screen is available on the computer system. In order to optimize, quantify, and store the data collected in a precise and exact manner, software for computer-controlled picture capture and editing should be provided. The system's built-in display or a second laptop that is attached to the system can do this.

From the aforementioned information, we learn about the many parts of a gel documentation system. Each component is necessary and has a purpose, helping to see and record gels produced by electrophoresis.

The gel documentation system is also useful for documenting 2-D electrophoresis, blotting method analysis, and post-translational changes.

Make contact with a reputable vendor like IGene Labserve if you intend to purchase a Gel Documentation System. Visit their official website at https://www.igenels.com for more information, or contact 09310696848 to chat with an expert.

Seven safety procedures for operating Laminar Air Flow

 

Laminar air flow is defined as airflow where the entire body of air within a space is uniform in velocity and direction. Users often miss out on some important details while operating laminar air flow. Here are the necessary processes to follow:

• It is important to make sure there are no UV-sensitive materials within the laminar flow cabinet before operating it.
• The UV light is then turned on after which the hood's glass shield is closed. To assure the working bench's surface sterilization, the UV light should be left on for about 15 minutes.
• Following the turning off of the UV light, the airflow is not turned on for approximately 10 minutes.
• The airflow is turned on 5 minutes before to the start of the procedure.
• Then, while doing the surgery, the glass shield is opened and the fluorescent light is turned on.
• The cabinet's work surface can be sterilized with additional disinfectants, such as 70% alcohol, to provide added protection.
• Following completion of the work, the glass shield, the fluorescent bulb, and the airflow are all shut off.

Laminar flow hoods are often used with clean benches and biological safety cabinets. They are containment chambers for laboratories with HEPA filtration for precisely aimed research. Some of these hoods guard objects put in the work area against contamination. The user is shielded from toxins in the work environment by other devices. Biological samples, semiconductors, and other delicate materials are frequently handled under laminar flow hoods.

Working Principle of PCR Workstation

 

The technique was created in the 1980s to make studying a specific piece of a DNA strand simpler, PCR was created in order to amplify or multiply that segment.

The use of PCR may be found in a variety of fields, including environmental research, medicine, and crime scene analysis.

·Prior to use, the whole hood should always be ethanol sterilized.
·Make sure there aren't any open flames within the hood.
·Anything that obstructs the air grills has to be taken out.
·The ventilator and HEPA filters need to be checked often to make sure they're operating at their best (99.99 percent efficiency at 0.3 microns)