Important things to know about Vertical Gel Electrophoresis

Proteins and nucleic acids (DNA or RNA) can be separated using gel electrophoresis according to their size. Labs use vertical electrophoresis systems to examine drugs, forensics, vaccinations, DNA profiling, or other life science applications.

Some experts say that the vertical gel electrophoresis method is thought to be more sophisticated than the horizontal gel electrophoresis method. It operates in accordance with the basic principle of gel electrophoresis. This method makes use of an intermittent buffer. The upper chamber houses the cathode, while the lower chamber houses the anode. The important electric field is produced by the electrodes found in each compartment. The two glass plates are separated by a small layer of gel.

As a result, the top half of the gel is immersed in the top chamber, while the bottom section is immersed in the bottom chamber. Following the application of the current, a small amount of the buffer passes through the gel from the top chamber to the bottom chamber. In this approach, very little electricity is used.

 Similarities Between Horizontal and Vertical Gel Electrophoresis

• Both techniques employ an anode and a cathode to create the necessary electric field.
• Both methods work according to the fundamental principles of gel electrophoresis.

Find out the Difference Between Horizontal and Vertical Gel Electrophoresis.

Horizontal vs Vertical Gel Electrophoresis

Horizontal- The gel is present in a horizontal position when using the horizontal gel electrophoresis method.

Vertical- Gel is positioned vertically during a process called vertical gel electrophoresis.

Buffer

Horizontal- A continuous buffer is used in horizontal gel electrophoresis.

Vertical- Vertical gel electrophoresis uses a discontinuous running buffer.

Use of Acrylamide

Horizontal- Due to ambient oxygen exposure in the gel box during horizontal gel electrophoresis, acrylamide cannot be used.

Vertical- The use of acrylamide for vertical gel electrophoresis is possible because the gel is protected from ambient oxygen by two distinct chambers.

Function

Horizontal- Although proteins cannot be separated by horizontal gel electrophoresis, DNA and RNA mixtures may.

Vertical- To separate protein mixtures, vertical gel electrophoresis is employed.

Buffer only passes across the gel during vertical gel electrophoresis. As a result, the voltage gradient during the separation step may be precisely controlled. Due to the compartments' lack of exposure to ozone from the atmosphere, acrylamide gel can be used. Acrylamide gel's smaller pore size makes it possible to separate substances precisely and with greater precision.

These are some of the most important things to understand about vertical gel electrophoresis and how it is different from horizontal gel electrophoresis. However different they are, both systems function according to the common principle of gel electrophoresis.

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