Types of Stability Chamber

 

Environmental chambers
Temperature – humidity chambers
Accelerated test chambers
Photostability chambers

Principles of Double Beam UV Spectrophotometer

A spectrophotometer is an optical device that implements the property of light rays to measure a particular wavelength. Hence, these instruments are also called analytical optical instruments. The prime use of a double beam UV spectrophotometer is to detect and confirm the presence of chemical species present within the biological specimens grounded on their chemical structures, concentration, etc. A double beam spectrophotometer uses the light rays in their visible wavelength and calculates in the same wavelength of light.

The double beam spectrophotometer emits the photons as light packets. These photons pass through multiple biological specimens where the light beam is absorbed for measuring the presence of biochemical entities. A spectrophotometer calculates the intensities of light at different wavelengths through their precise instrumentation.

What are the principles of a double beam UV spectrophotometer?

A double beam UV spectrophotometer works on the same principle as the single beam spectrophotometer. However, there are some additional phenomena of light rays in the UV spectrophotometers. The working principles of a double beam spectrophotometer are listed below:

1. Dispersion of light rays: You can see the dispersion of light rays in nature just after the rain. The droplets diffract the light rays of the sun by generating a spectrum of color. In this case, the spectrum is termed the rainbow. The same principle applies in the double beam UV spectrophotometer where the diffraction of light happens through the specimens. This refraction of light makes the spectrophotometry process more reproducible. In this equipment, the diffraction angles rely on the wavelength of the main incident light.
2. Absorbance: It is defined as the ratio of incident light to transmitted radiant light rays through a biological sample during the process of spectrophotometry. The cells contained in the samples reflect the light and also absorb the same that gets transferred to the detectors present in the spectrophotometer. When the case is of biological samples that scatter light, absorbance can be defined as the ability of the sample to absorb more and more light passing through it.
3. The visible spectrum scattering of light rays: In a double beam spectrophotometer, when white light passes through the sample and is reflected by a colored object, a certain portion of the wavelengths is absorbed. The remaining light beam will be guessed as the complementary color to the wavelength of light absorbed. It is demonstrated by the color spectrum.

The double beam UV spectrophotometer analyzes different biological specimens for good results. The electromagnetic spectrum is also implemented in the process of making measurements out of samples. Every process relating to dispersion, reflection, and refraction happens in spectrophotometry which further helps in making analytical measurements.

Important things to know about Vertical Gel Electrophoresis

Proteins and nucleic acids (DNA or RNA) can be separated using gel electrophoresis according to their size. Labs use vertical electrophoresis systems to examine drugs, forensics, vaccinations, DNA profiling, or other life science applications.

Some experts say that the vertical gel electrophoresis method is thought to be more sophisticated than the horizontal gel electrophoresis method. It operates in accordance with the basic principle of gel electrophoresis. This method makes use of an intermittent buffer. The upper chamber houses the cathode, while the lower chamber houses the anode. The important electric field is produced by the electrodes found in each compartment. The two glass plates are separated by a small layer of gel.

As a result, the top half of the gel is immersed in the top chamber, while the bottom section is immersed in the bottom chamber. Following the application of the current, a small amount of the buffer passes through the gel from the top chamber to the bottom chamber. In this approach, very little electricity is used.

 Similarities Between Horizontal and Vertical Gel Electrophoresis

• Both techniques employ an anode and a cathode to create the necessary electric field.
• Both methods work according to the fundamental principles of gel electrophoresis.

Find out the Difference Between Horizontal and Vertical Gel Electrophoresis.

Horizontal vs Vertical Gel Electrophoresis

Horizontal- The gel is present in a horizontal position when using the horizontal gel electrophoresis method.

Vertical- Gel is positioned vertically during a process called vertical gel electrophoresis.

Buffer

Horizontal- A continuous buffer is used in horizontal gel electrophoresis.

Vertical- Vertical gel electrophoresis uses a discontinuous running buffer.

Use of Acrylamide

Horizontal- Due to ambient oxygen exposure in the gel box during horizontal gel electrophoresis, acrylamide cannot be used.

Vertical- The use of acrylamide for vertical gel electrophoresis is possible because the gel is protected from ambient oxygen by two distinct chambers.

Function

Horizontal- Although proteins cannot be separated by horizontal gel electrophoresis, DNA and RNA mixtures may.

Vertical- To separate protein mixtures, vertical gel electrophoresis is employed.

Buffer only passes across the gel during vertical gel electrophoresis. As a result, the voltage gradient during the separation step may be precisely controlled. Due to the compartments' lack of exposure to ozone from the atmosphere, acrylamide gel can be used. Acrylamide gel's smaller pore size makes it possible to separate substances precisely and with greater precision.

These are some of the most important things to understand about vertical gel electrophoresis and how it is different from horizontal gel electrophoresis. However different they are, both systems function according to the common principle of gel electrophoresis.

If you are looking to buy a quality vertical gel electrophoresis, connect with IGene Labserve by visiting https://www.igenels.com/ or dial 09310696848 to understand everything about the product.

Most Recommended Products of Igene Labserve

 

Laminar Air Flow
Biosafety Cabinet
PCR machine
Gel Documentation System
PCR Workstation
Stability Chamber
Laboratory Fume Hoods
Ultra Low Temperature Freezer
Double Beam UV spectrophotometer
Vertical Gel Electrophoresis System

Lab Freezers: Types, Qualities, and Recommended Use

 

Freezers are one of the reasons behind the successful operation of various laboratories. Freezers provide dependable and more importantly, safe storage conditions for materials demanding a low-temperature environment. There are different freezers to choose from based on the needs of a specific lab. Specific lab freezers address particular needs within the laboratory and medical environments.

Types of lab freezers

Given below are some of the types of lab freezers:

• Ultra-low temperature freezers

Qualities

If your research lab comprises materials that demand an environment with low temperatures, the best option will be to acquire ultra-low temperature freezer. With these freezers in place, you can store the samples and materials below -40⁰C. It is even possible to achieve temperatures as low as -86⁰C since some ultra-low freezers contain two independent evaporator circuits revolving around the interior chamber. An ultra-low temperature freezer uses a significant amount of energy to maintain the lowest temperatures.

Recommended Use

Ultra-low temperature freezer are apt for the storage of materials requiring low temperatures in medical establishments and labs.

• Laboratory deep freezer

Qualities

These freezers are available with a temperature range from -5⁰C to -20⁰C based on the model. The presence of a powerful cooling machine and special insulation ensures quick temperature stabilization after opening the door of the laboratory deep freezer. Hence, your samples are always stored securely. It comes with a microprocessor controlled by an alarm and an LED display. The build-in voltage stabilizer ranges from 220-230V. More often, a laboratory freezer has two adjustable feet and four casters. There is a provision for resetting the control panel to zero for validation.

Recommended Use

Laboratory deep freezers play a great hand in the safe storage of frozen vaccines, reagents, and other temperature-sensitive specimens for research needs.

Generally, deep freezers are the testing accessories that are used to preserve and store blood samples, food products, medical equipment, and injections for an extended period. Deep freezers are used both for industrial and household purposes. The technical specifications and functions of the equipment vary according to the requirements of the test application.