Make many copies of small segment of DNA
Molecular Photocopying - Amplify DNA enough to study in detail
DNA produced by PCR has a variety of uses
Working Principle
Denaturing
Annealing
Extension/Elongation
DNA template
DNA Polymerase
Primers
dNTPs(Deoxynucleotide triphosphate)
PCR Buffer
Types of PCR
Conventional PCR machine
qPCR
Reverse Transcription
Nested
Hot Start
Digital (dPCR) (Droplet Digital PCR (ddPCR) / qdPCR)
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