One of the most significant and advanced molecular biology instruments, the PCR Machine has created a new chapter by revolutionizing DNA study. Kary B. Mullis was awarded a Nobel Prize for his invaluable discovery and contribution to Chemistry.
After amplification from the machine, the DNA is used in various lab procedures like human genomic projects (HGP), laboratory and clinical techniques that include DNA fingerprint, bacteria detection, and genetic disorder diagnosis.
How is the amplification of DNA segment done in PCR?
After heating the sample to denature DNA or separate the two strands of DNA into a single strand. With an enzyme, "Taq polymerase" synthesizes building new DNA strands from the original strands that are termed templates. With this process of duplication from the templates, each molecule contains an old and new DNS strand. Each strand creates two new copies and the process goes on. This cycle is repeated 30-40 times of synthesized and denatured DNA producing more than a billion copies from the original segment. The machine works in an automated format and is capable of completing the procedure in a few hours; the thermocycler machine can alter the temperature automatically as it is programmed to denaturing and synthesize every minute.
What are the requirements that your system must have?
A single PCR is unable to accommodate the requirements of the research community. For genomic application, an optimally configured rtPCR device is good for most laboratories. The designs are varied with Peltier elements accommodating tubes and microtiter plates. For countering the “edge effects” of achieving different temperatures, different approaches are used in overcoming the problem. In some cases independent thermal electric modules monitor each sample in different phases; in some machines, a carousel system spins the sample in temperature-controlled cabins maintaining equalized thermal stability.
Some companies work on closed systems using specific reagents or reaction vessels/ kits. This makes the entire assembly expensive and with limited flexibility. The reaction speed of the machine matters, some machines have robust throughput allowing standard rtPCR reaction that can be completed in half an hour to a little less than an hour producing 384 well plates.
Conclusion
Standalone PCR systems computer-controlled with user interface come with data storage facilities and network options. A careful study of what you require from your PCR machine is necessary before you select the right instrument. Meeting your specific laboratory requirements and pricing considerations, iGene Labserve brings the most convenient closed cabinet-fitted HEPA filtered airflow systems that maintain minimum turbulence with maximum output. Visit https://www.igenels.com/ for carrying out specialized work in a bacteria-free environment.
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