A quick overview of the Gel Documentation System

 

Gel Documentation System measures and records labeled protein and nucleic acid; different media like acrylamide, cellulose, or agarose are used for the diagnosis. The equipment aids in quick evaluation after electrophoresis which proves to be economical in terms of chemical and time. You can see the results in less than five minutes through stain-free technology. This testing equipment is used in research labs that help you to visualize photo-documented nucleic acid separated samples taking place after electrophoresis, show microbial colonies count and protein separation on western blots, and thin layer chromatography (TLC) mixture identification. 

What is the basic working of a Gel Imager?

The gel imager comes with an ultraviolet (UV)/ visible (blue or white) transilluminator. The light source is blocked from penetrating with a hood and inside the equipment; a high-resolution camera captures the image. The procedure involves samples mixed with the agarose gels placed on a Petri dish or TLC films, placed on the transilluminator surface. The door closes down and prevents the UV light from penetrating outside harming the user. Once the lamp is powered the photo documenting through CCD and CMOS cameras takes place. The images can be retrieved and exported on Microsoft Excel.

Labs that use the gel images

Used in molecular biology labs for genomics, proteomics, the imaging system identifies PCR segments with DNA quantification, bacterial cell culture, protein separation, and environmental sample testing. Emitting visible light or Ultra Violet light for visualizing chemiluminescent samples or bacterial sample count, the UV light should match the absorption spectrum of the fluorescent dye used at the time of gel separation through a blotting and thin chromatography process. For colony counting at its optimal amount, the blue light emitted at 470 nanometers and staining samples through commercial dye commonly use SYBR Green, SYBR Gold, or SYBR Safe. Visualize, UV 254 nanometer light at its optimal amount to identify DNA cross-linking, for short exposure gels, stained with Ethidium Bromide 302 nanometer light is best suited and for gel band cutting, 365 nanometer light suits perfectly.

Gel Documentation System Application and uses

B1 - horizontal minigel system

For identifying DNA and RNA samples for analysis, cloning, and sequencing, samples of DNA and RNA (Necluic acids)are loaded on agarose or acrylamide gel. Exposing them to the electric field, the negatively charged samples travel towards the positive electrode. The smaller DNA and RNA fragments travel faster than the longer ones, enabling fragment identification aided by the fluorescent dyes. Once identified, the samples are separated for purification.

 B2 - horizontal minigel system

The protein sample is immersed in Tris-Glycene or Tris-Acetate– Tris-Glycene or Tris-Acetate which is commercially available and is effective in purification and separation. The protein fragments travel at different speeds through the gel metrics. Mass spectrometry, sample denaturing, and blotting use the protein gel.

B3 Colony counting

Microbial colony-forming units’ optimal growth is determined within the petri dish that has cell media loaded in it.

B4 Thin layer Chromatography

Quantification and qualification of a mass of substances include lipids, carbohydrates, fatty acids, and pesticides.

B5 Southern and Western Blot Gel

Separates proteins and isolates them for purification of active gels and SDS-PAGE gels.

Southern Blotting Gels

Separation of DNA fragments from blood and tissue samples restricting enzyme digestion through polyacrylamide (PAGE) gels and sodium dodecyl sulfate (SDS) gels combining urea.

Conclusion

A necessity for any lab that demands protein analysis, the imaging system comes in a range of specifications. However, you can customize the level of application if you order your gel imager from iGene Labserve. Visit our website and get in touch for details https://www.igenels.com/. You can also explore our gallery of scientific equipment to expand your lab assets.

What to expect from your PCR Machine?

 

One of the most significant and advanced molecular biology instruments, the PCR Machine has created a new chapter by revolutionizing DNA study. Kary B. Mullis was awarded a Nobel Prize for his invaluable discovery and contribution to Chemistry.

After amplification from the machine, the DNA is used in various lab procedures like human genomic projects (HGP), laboratory and clinical techniques that include DNA fingerprint, bacteria detection, and genetic disorder diagnosis.

How is the amplification of DNA segment done in PCR?

After heating the sample to denature DNA or separate the two strands of DNA into a single strand. With an enzyme, "Taq polymerase" synthesizes building new DNA strands from the original strands that are termed templates. With this process of duplication from the templates, each molecule contains an old and new DNS strand. Each strand creates two new copies and the process goes on. This cycle is repeated 30-40 times of synthesized and denatured DNA producing more than a billion copies from the original segment. The machine works in an automated format and is capable of completing the procedure in a few hours; the thermocycler machine can alter the temperature automatically as it is programmed to denaturing and synthesize every minute.

What are the requirements that your system must have?

A single PCR is unable to accommodate the requirements of the research community. For genomic application, an optimally configured rtPCR device is good for most laboratories. The designs are varied with Peltier elements accommodating tubes and microtiter plates. For countering the “edge effects” of achieving different temperatures, different approaches are used in overcoming the problem. In some cases independent thermal electric modules monitor each sample in different phases; in some machines, a carousel system spins the sample in temperature-controlled cabins maintaining equalized thermal stability.

Some companies work on closed systems using specific reagents or reaction vessels/ kits. This makes the entire assembly expensive and with limited flexibility. The reaction speed of the machine matters, some machines have robust throughput allowing standard rtPCR reaction that can be completed in half an hour to a little less than an hour producing 384 well plates.

 

Conclusion

Standalone PCR systems computer-controlled with user interface come with data storage facilities and network options. A careful study of what you require from your PCR machine is necessary before you select the right instrument. Meeting your specific laboratory requirements and pricing considerations, iGene Labserve brings the most convenient closed cabinet-fitted HEPA filtered airflow systems that maintain minimum turbulence with maximum output. Visit https://www.igenels.com/ for carrying out specialized work in a bacteria-free environment.

All About Vertical Gel Electrophoresis System

 

What is it
Types of
Benefits
Components
Descriptions
Instrument Used
Uses
https://www.igenels.com/product/vertical-gel-electrophoresis-system/

Which Biosafety Cabinet type is right for you?

 

Biosafety Cabinet (BSC) is a laboratory-controlled lab-engineered enclosure that protects samples, lab workers, and the laboratory environment against exposure and biohazards, splashes, and aerosols. The chances of material splashes and aerosols arise from material handling and manipulation of highly communicable agents. It is necessary to periodically check the efficiency of safety cabinets to verify the airflow, HEPA integrity, and how well the instrument is being able to protect the sample stored inside the chamber. All these factors depend on the cabinet in use.

The Biological safety cabinet comes in three classes. Class I cabinets, Class II cabinets, and Class III cabinets. Let's go through a quick review of what each entails.

Class I cabinet

This is a simple cabinet that provides personnel and environmental safety. However, in this type of cabinet, the product is subject to contamination. The airflow suctioned inside the cabinet is discharged through the HEPA filters.

Class II cabinet

This ventilated cabinet provides both personnel, environmental, and product safety. Found in research and clinical labs this is ideal for labs that deal with infectious agents. This cabinet is further divided into four three risk groups. A1, A2, B1, B2 are the different types of class II cabinets that are differentiated by the ratio of their exhaust and air recirculation.

Class III Cabinets

This cabinet provides the highest level of safety and is used extensively for protecting the most transmissible agents. These cabinets are used in Level 3 and 4 laboratories. These are completely enclosed cabinets that are leak proof, with HEPA filtered air. The operator's access is provided using glove boxes that enable work in the enclosed workspace. Dedicated exhaust system exterior maintains an interior negative pressure.

Conclusion

Providing the most technologically advanced Biosafety Cabinet iGenge labserve brings the most advanced laboratory equipment that increases lab experiment success and opens scope to major and delicate investigations in the field of bioscience. To explore our product brochure visit https://www.igenels.com/