Procedure and uses of the gel documentation system

 

Gel documentation system uses ethidium bromide, a fluorescent substance bound to nucleic acid. The ultraviolet rays excite fluorescent light. Binding itself to the nucleic acid the fluorescent substance concentration depends on the molecular weight of the nucleic acid. luminosity is larger in molecules that have more weight. All large samples will shine out conversely smaller substances show a tiny quantity of fluorescence.

when a double-strand DNA is bound by ethidium bromide, the structure displays hydrophobic rings present in DNA. Close van der Waals interactions are formed with the base pair and untwist the helix. The principle of using ethidium bromide as a transilluminator through the technique of agarose gel electrophoresis shows protein and nucleic acids.

In genetic engineering, the use of ethidium bromide helps in detecting nucleic acids in microbiology labs with Gel documentation system. The system helps in detecting single-strand RNA and double-strand DNA from PCR restriction digest. The detection process involves a gel containing nucleic acids under UV lights. The rays are harmful to the eyes and skin. Ethidium bromide acts as a mutagen based on the time of exposure. Viewed indirectly through enclosed cameras and images capturing fluorescent photographs on computer screens. For direct viewing, adequate protection with glasses and skin protection must be worn.

iGeneLabserve’s gel doc system is highly efficient in detecting protein and nucleic acids in DNAs and RNAs. The protection and caution instructions are served with the equipment manual. Visit https://www.igenels.com/ for details.