The risk of contracting diseases among lab workers is quite high as they deal with biologically hazardous infections causing viruses and bacteria. The risk of disease is 8,000 times higher in lab technicians, especially when dealing with highly sensitive communicable diseases. It is imperative to provide a safe working enclosed cabinet where they can work with safety. The Biosafety Cabinet serve this purpose and safeguard the technician from exposure to harmful and disease-causing microorganisms.
Guarding lab workers against biohazardous particles, labs dealing with such materials must have a biosafety cabinet installed in their laboratory immediately if they already do not have one. While working on highly transmissible particles they should wear proper PPE (personal protective equipment) for no exposure to these particles.
What are the three classes of BSC?
BSC come in three classes, Class I, Class II and Class III cabinets. All three perform a common function of protecting the sample enclosed within the cabinet. Let us understand each’s function in detail.
Class I
This BSC features a sash which is the window and the only connection that the investigator has with the samples inside the cabinet. As soon as the sash is parted an inward surge of air blows into the cabinet to prevent aerosol movement of the particles outside through negative pressure. This air movement protects the person and the surrounding environment of the lab. However, the sample inside the cabinet may be contaminated by the inward unfiltered air circulation.
Class II
This cabinet solves the problem faced by the first cabinet. Through a double directional airflow, it creates an air barrier between the lab and the sample. The shield prevents the outside air to reach the sample. The air maintains a downward flow within the cabinet from the drawn-in air which is sucked by the HEPS filters. This cabinet is further classified in type A1, A2, B1 or B2 cabinets depending on their air circulation systems, and air exhaust designs.
It should be noted that both Class I and II are prone to disruption and should be placed close to open windows and in secluded lab areas.
Class III
This is a fully closed cabinet with only glove port inlets. The ports allow work inside the cabinet. An interlocking door at the side of the work area allows the transfer of materials. The air streaming works just like the class II cabinet system protecting the aerosol transfer of transmissible microorganisms, the air is efficiently exhausted by the HEPA filters.
Conclusion
If you are looking for the best-in-class Biosafety cabinets then iGeneLabserve is a company that you can safely choose for high in technology laboratory equipment. Creating safe working environments for lab workers and at the same time making scientific findings possible their instruments are of international quality. Visit https://www.igenels.com/ to view their product portfolio and place your order.
When dealing with highly communicable lab samples that demand ventilated enclosure laboratory fume hoods is the most useful equipment. It separates sensitive contaminated samples with a different ventilation and air conditioning system not related to the building’s HVAC system preventing the recirculation of the air back into the building.
When you are working on toxic compounds and compounds with a boiling point below 120 degrees Celsius, laboratory fume hoods are a must. These are effective local ventilation equipment that controls exposure to materials through enclosed lab conditions.
Fume food controls include
Constant air volume (CAV)
Variable air volume (VAV)
Two state (two speed) control
In the older versions of fume hoods, fume cupboards can still be found.
Fume hoods are grouped into two categories.
The first category belongs to the low standard flow fume food that is designed to operate at 100 linear feet per minute. These maintain an 18 inches sash height.
What should you know when using a fume hood?
There is a possibility of exposure and air contaminants generated by laboratory activity. In such a situation a fume hood rather than a benchtop is more reliable in avoiding any sort of overexposure and keeping your surroundings guarded against highly sensitive materials avoiding room contamination. When there is a requirement for service or repair, lab researchers and personnel should supervise the decontamination of the fuel food and ensure that the ventilation system is shut down. When the room ventilation system is shut down, the fume food must not be used.
What needs to be kept in mind when installing and replacing fume hoods?
When installing a fume hood, you need to follow a design and planning which includes, decontamination, asbestos abatement, and many such pre-installation activities that make the installation process safe and effective. You may need the help of a technician from the company to assist you with the installation of the hood system. When purchasing your hood from iGeneLabserve, https://www.igenels.com/ you will find complete assistance from the team to install your equipment. Connect today.
Gel documentation system uses ethidium bromide, a fluorescent substance bound to nucleic acid. The ultraviolet rays excite fluorescent light. Binding itself to the nucleic acid the fluorescent substance concentration depends on the molecular weight of the nucleic acid. luminosity is larger in molecules that have more weight. All large samples will shine out conversely smaller substances show a tiny quantity of fluorescence.
when a double-strand DNA is bound by ethidium bromide, the structure displays hydrophobic rings present in DNA. Close van der Waals interactions are formed with the base pair and untwist the helix. The principle of using ethidium bromide as a transilluminator through the technique of agarose gel electrophoresis shows protein and nucleic acids.
In genetic engineering, the use of ethidium bromide helps in detecting nucleic acids in microbiology labs with Gel documentation system.
The system helps in detecting single-strand RNA and double-strand DNA
from PCR restriction digest. The detection process involves a gel
containing nucleic acids under UV lights. The rays are harmful to the
eyes and skin. Ethidium bromide acts as a mutagen based on the time of
exposure. Viewed indirectly through enclosed cameras and images
capturing fluorescent photographs on computer screens. For direct
viewing, adequate protection with glasses and skin protection must be
worn.
iGeneLabserve’s gel doc system is highly efficient in detecting protein and nucleic acids in DNAs and RNAs. The protection and caution instructions are served with the equipment manual. Visit https://www.igenels.com/ for details.
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