How Bead Beater Homogenizers Improve Protein Yield and Minimize Sample Degradation?

 

Protein extraction is one of the most crucial steps in various biotechnological and biochemical research in laboratories. The aim of this procedure is extracting proteins from biological samples while maintaining their functionality and integrity for further analysis. That is how bead beater homogenizers have emerged as an essential tool in the process of protein extraction. The efficiency of a bead beater homogenizer is revolutionizing the field of biotechnology to a great extent.

Let us find out how a bead beating homogenizer improves the efficiency of protein extraction:

1.      Mitigating  contamination of samples

Any research worker is familiar with how sample contamination can affect the reliability and quality of protein extraction. Bead beating homogenizers are highly effective for cell disruption within a closed system, thereby reducing cross-contamination between samples. Usually, homogenizers have single-use tubes for running the operations and reduce the chances of contamination greatly. That way, homogenizers are perfect for all those applications where sample integrity and purity plays a vital role. No wonder these devices are worthy of use in high-precision experiments and proteomics.

2.      Disruption of cells

One of the major challenges of protein extraction is breaking the cells to allow them to release proteins. With bead beating homogenizers, you can expect mechanical agitation occurring at the highest speed and create maximum impact by shaking small beads within the sample. When these beads confront and collide with the cell, it leads to mechanical disruption of membranes and cell walls. In this manner, its efficiency becomes noticed when breaking tough cell walls in bacterial samples as well as cell walls in plants. Moreover, cell lysis efficiency also improves radically, ensuring maximum yield of proteins. However, you need to follow bead beater homogenizer protocol for desired outcome.

3.      Versatility and scalability

Bead beating homogenizers are available in a wide range of models to process different sizes of samples. So, whether it is for large or small quantities of samples needed for laboratory applications, you just need to check if it is the right model to sit your needs. 

Due to versatility and scalability of the right model of bead beating homogenizer, it has come to be one of the most indispensable tools in various production settings and laboratories. No matter what your requirements are for processing the smallest amount of protein or up to several litres of samples, bead beaters are capable of producing the most efficient results. For same sample sizes, the model chosen ensures high level of standardization.

4.      Consistency of results

Achieving consistent results is the key to any scientific process. Bead beating homogenizers make the disruption process uniform for varied range of samples, ensuring consistency during the extraction of proteins. Wondering how? The mechanical force that the device applies on the samples is distributed evenly, allowing it to create the best results. The perfect blend of bead size, time, and speed can be fine-tuned in this device to boost the process of extraction. Due to this consistency of results, the bead beater homogenizer proves its usage in clinical and research settings in the field of biotechnology.

5.      Time is of essence

Just like several other processes in the field of biotechnology, bead beating homogenizers can rev up the protein extraction process. However, you cannot achieve the desired outcome unless you understand the significance of time. That is why bead beating homogenizers are being extensively used today; the fast mechanical action of the beads moves ahead for faster protein release and lysis, reducing the processing time greatly. The enhanced efficiency level can be greatly reduced in high-throughput applications where sensitivity of time plays a significant role. Researchers too are keener to use this device in place achieve time-sensitive results when process a large number of samples in record time.

6.      Reduces protein degradation

Degradation of proteins poses a serious risk during protein extraction, resulting in the loss of valuable samples. The advantage of using the bead beating homogenizer is that it helps adjust the intensity and speed of the beating process, allowing researchers break the cells.

This is especially important to ensure that the proteins remain intact during the process of homogenization. It serves as a perfect device during isolation of sensitive proteins where preservation of downstream applications is crucial like in mass spectrometry, Western blotting, and enzyme assays.

Bead beater homogenizers have transformed the way protein extraction takes place in laboratories.  Undoubtedly, it improves the efficiency of protein yield with high scalability and processing times. With the rising demand of high-quality protein extraction, it’s time to explore bead beater homogenizer price to get the right device for your laboratory. IGene Labserve is one of the most trusted places to order the best-quality laboratory equipment. Navigate here to explore the specifications of various models and create a revolution in proteomics.

3 Common Fume Hood Errors You Must Avoid to Ensure Lab Safety

 

Fume hoods is among the safety devices used in laboratory settings. Unfortunately, even the best make and model of laboratory fume hoods may fall short of efficiency and safety when you fail to comply with the safety protocols within the laboratory. Keep in mind that the slightest mistake in handling the fume hood may lead to dangerous consequences.

So, here is a lie up of the fume hood mistakes and what you should do about them:

1.      Not having the training to handle the fume hood

You might have been working in the laboratory for a long time ad have a wealth of knowledge about the device and how they run. However, that does not certify that you are good to handle the fume hood as well. Anyone handling this device needs to have adequate training. That way, they won’t put the samples as well the workspace exposed to risks. If you have new people joining the laboratory research team, make sure to train them adequately and give them necessary instructions about mishandling the equipment.

2.      Not maintaining the required distance from the fume hood

One of the major lab safety errors that occur when working with a fume hood is not maintaining a safe distance from the fume hood.  In the event of fire or explosion, the people working near the fume hood are prone to sustain maximum injuries. A laboratory must not allow more people to stay near the fume hood, especially near the opening. A good way to prevent accidents would be placing the hood face in a location so that people don’t need to come in front of it.

Apart from this, you must ensure that things are not taken out of the hood and no ventilation is directed toward it like the HVAC ductwork or portable fans. It might disrupt the airflow and allow the contaminants to come out and cause exposure. Even the chemicals used need to be kept at a safe distance about six inches away from the face opening to maximise safety.

3.      Not testing the fume hood regularly

You need to follow several guidelines and comply with the safety standards when working with the hood. Among the most important safety protocols you need to follow is not testing the hood regularly. With this, you can ensure that the equipment is in good working order, capable of complying with the safety regulations, and can safeguard the workers as well as the quality of the samples. It is not hard to avoid these mistakes; all that is needed is proper knowledge for protecting laboratories and handling the fume hood.

4.      Storing chemicals inside the hood for  long period

You can store chemicals inside the laboratory fume hood when it is in operation but they are conventionally not designed for storing chemicals. When items are kept within the hood, it can cause unnecessary turbulence. As a result, the contaminants may spill out of the equipment. Typically, the airflow that comes out of the hood is stronger than what is required for storing closed containers. If you have concerns about storing chemicals, it needs to stay within safety cabinet that are approved for this purpose.

5.      Handling emergencies

Safety of fume hoods is crucial and is centred on technical expertise. Laboratory worker need to have proper training and education to keep mistakes at bay. However, accidents may still occur and pose risks to workers. Fr instance, if an employee notices chemical spills coming out of the equipment, the matter is to be immediately reported to the supervisors and everyone who are in charge of the laboratory. The first thing to do is deactivation of the device immediately when you notice signs of trouble.

Fume hoods are an integral part of most laboratories. So, using it properly ensures that it operates efficiently and the lab spared of risks that may lead to accidents. Every laboratory worker needs to ensure safe working environment in the laboratory and avoid the basis errors in handling the equipment.

Aare you looking forward to ordering a new fume hood that is safe and reliable for laboratory usage?  At IGene Labserve, we manufacture top-grade lab equipment and cater to a wide clientele. Get in touch with us here https://www.igenels.com/ to explore laboratory fume hoods and place the order today.