What is the difference between Vertical and horizontal Gel Electrophoresis?

 

Gel electrophoresis is a method for separating proteins and nucleic acids (DNA or RNA) based on their molecular weight. Forensics, immunizations, DNA profiling, and other applications in the life sciences are all examined in laboratories using vertical electrophoresis equipment.

According to some professionals, the vertical gel electrophoresis method is considered to be more advanced than the horizontal gel electrophoresis approach. It works according to the fundamentals of gel electrophoresis. This technique takes use of an erratic buffer. The cathode is located in the top chamber, while the anode is in the lower chamber. The electrodes present in each compartment create the crucial electric field. A thin gel layer lies between the two glass plates.

Difference between Vertical and horizontal Gel Electrophoresis

Vertical Gel Electrophoresis Horizontal Gel Electrophoresis Used to separate nucleic acids (50 – 20,000 bp) Used to separate proteins (5 – 250,000kDa) Utilize agarose gel Utilize polyacrylamide gel Run under native condition Run under denaturing condition Only 1 gel Two gel layers: stacking gel and resolving gel Relatively easy to set up Relatively more difficult to set up Ethidium bromide staining for DNA Coomassie or silver staining for proteins   Producing Reliable Results The key to successful gel electrophoresis is to optimize the operating parameters, including voltage, buffer selection, buffer pH, dye, appropriate reference marker, and more. Any of the aforementioned elements may have an impact on how clearly the gel electrophoresis result is resolved. A matrix's pore size is determined by the concentration of the polyacrylamide or agarose gel, which is crucial. A result can only be obtained if the sample size can pass through the pore size. For biological molecules whose charge does not change, voltage, in addition to pore size, controls the rate of migration. Thus, you may adjust the voltage and gel % to acquire the best electrophoresis results. It's also crucial to select a buffer that works for your experiment's design. Applications that will be made following the electrophoresis process are also a crucial factor to take into account when choosing the appropriate buffer. Regardless of whether you use a protein ladder or a nucleic acid reference marker, it is crucial to have even the tiniest indication of the expected product size. The assessment of product size will be challenging if a ladder that is beyond the product range is used. When separating mixtures including DNA, RNA, and protein molecules, gel electrophoresis is a common scientific technique utilized. Gel electrophoresis may be done in two different ways: vertical and horizontal. Whereas the running buffer is discontinuous in vertical gel electrophoresis, it is continuous in horizontal gel electrophoresis. Vertical and horizontal gel electrophoresis differs in this way. The common concept of gel electrophoresis underlies how both systems operate. If you are looking to buy a reliable vertical gel electrophoresis, connect with IGene Labserve by visiting https://www.igenels.com/ or dial 09310696848 to understand everything about the product.

Understanding PCR Workstation: A Complete Guide

 

PCR (polymerase chain reaction) is a widely used molecular biology technique that amplifies DNA segments. It has become an essential tool in various fields, including medical research, genetic testing, and forensic science. PCR requires a sterile environment to prevent contamination and false results, and that is where PCR workstations come in.

PCR workstation are specialized laboratory equipment designed to provide a contamination-free environment for PCR experiments. They use HEPA filters and UV lights to eliminate unwanted particulates and microorganisms from the workspace. In this blog post, we will discuss PCR workstations, their types, features, and applications.

Types of PCR Workstations

PCR workstations come in various types, each with its unique features and benefits. Here are some common types of PCR workstations:

Vertical PCR Workstations
Vertical PCR workstations are the most common type of PCR workstation. They use a vertical laminar airflow to create a sterile environment. The airflow passes through a HEPA filter, removing any particulate and microorganism present in the workspace. Vertical PCR workstations are compact and easy to use, making them ideal for small laboratories.

Horizontal PCR Workstations
Horizontal PCR workstations are designed to provide a sterile environment for PCR experiments while maximizing workspace. They use a horizontal airflow, which is ideal for large samples and equipment. The workstation's HEPA filter removes unwanted particulates and microorganisms from the workspace. Horizontal PCR workstations are spacious, making them ideal for large laboratories.

PCR Cabinets
PCR cabinets are the most basic type of PCR workstation. They provide a closed-off space for PCR experiments, reducing the risk of contamination. PCR cabinets do not use HEPA filters or UV lights, making them less expensive than other types of PCR workstations.

Features of PCR Workstations

PCR workstations have various features that make them ideal for PCR experiments. Here are some common features of PCR workstations:

HEPA Filters
HEPA filters are essential in PCR workstation. They remove any particulate and microorganisms present in the workspace, preventing contamination of the PCR samples.

UV Lights
UV lights are used to sterilize the workstation's surfaces, preventing the growth of microorganisms. They are also used to disinfect the air passing through the workstation's HEPA filter.

Digital Display
PCR workstations often come with a digital display that shows the workstation's temperature, humidity, and airflow rate. This feature is essential in maintaining the ideal environment for PCR experiments.

Applications of PCR Workstations

PCR workstations are used in various fields, including medical research, genetic testing, and forensic science. Here are some common applications of PCR workstations:

Medical Research
PCR workstations are used in medical research to amplify DNA segments for genetic testing and analysis. They are also used to study infectious diseases and develop vaccines.

Genetic Testing
PCR workstations are used in genetic testing to identify genetic disorders and mutations. They are also used in paternity testing and forensic science.

Forensic Science
PCR workstations are used in forensic science to analyze DNA samples for criminal investigations. They are also used to identify human remains and establish biological relationships.

Conclusion

PCR workstations are essential laboratory equipment in PCR experiments. They provide a contamination-free environment, preventing false results and inaccurate data. PCR workstations come in various types, each with its unique features and benefits. They are used in various fields, including medical research, genetic testing, and forensic science. If you're looking for a reliable PCR workstation, consider the type of PCR experiments you'll be performing and choose a workstation that meets your needs.