The Indispensable Role and Diverse Applications of Bacteriological Incubators in Modern labs

 

Bacterial incubators play a significant role in enabling microbiologists, clinical researchers, and scientists conduct controlled experiments in laboratories. They are specially designed to maintain stable temperatures in the environment and are naturally indispensable for the growth and cultivation of microbial cultures.

Here is why a bacteriological incubator is a must-have in the modern labs and their applications:

• Precision and reproducibility

Consistency is the key factor in any scientific research. That is why the microbiology incubator is considered vital for maintaining precise temperature conditions that are crucial for repeated experiments. Any variation of temperature in this sequence can alter the growth rates of microbes and impact the metabolic activity resulting in flawed outcome.

• Compliance with research standards

The regulatory bodies like FDA, WHO, and others demand controlled environments for testing in clinical and pharmaceutical research. That way, they help laboratories meet quality and compliance standards.

Top applications of bacteriological incubators

Here are the primary applications of a bacteriological incubator:

1. Microbiological research

Universities and research institutions rely on bacteriological incubators to a great extent for:

• Studying bacterial growth kinetics
• Analyzing the impact of antibiotics on various strains
• Observing colony morphology and pigmentation
• Performing biochemical tests

Student labs also use incubators in basic microbiology practical, making them important for educational purposes as well.

2. Cultivation of microbes

Incubators aid in the growth of yeast, bacteria, and fungi, allowing researchers to study their behaviors, characteristics, and fungi as well as their responses to different stimuli. Such data obtained from lab incubator for bacteria is extremely useful in the field of biotechnology, microbiology, and environmental science.

3. Genetics and molecular biology

Incubators play a crucial role for performing polymerase chain reactions or PCR and hybridization of DNA. The ability to control temperature ensures the efficiency of these processes, allowing researchers to analyze and manipulate DNA sequences.

4. Environmental monitoring

Public health labs use bacteriological incubators to test environmental samples for:

• Assessing microbial contamination in drinking water
• Evaluating wastewater treatment efficiency
• Studying soil bacteria or bioindicators in environmental studies

5. Protein purification and expression

In molecular biology, incubators express and purify proteins that are fundamental in assessing cellular functions and in the development of therapeutic agents.

6. Biotech and pharmaceutical industries

In the pharmaceutical world, incubators are critical in:

• Sterility testing for drugs and injectables
• Assessing microbial contamination levels
• Vaccine development by supporting bacterial culture needed for antigen production
• Supporting R&D for new antibiotics or probiotics

Biotechnology labs use incubators for fermentation experiments, recombinant DNA work, and enzyme production through microbial expression systems.

A modern microbiological incubator comes equipped with features that enhance efficiency, safety, and control:

• Digital temperature controls for precise adjustments
• Programmable timers and alarms for automated operations
• UV germicidal lamps to maintain internal sterility
• Interior glass doors to monitor cultures without disrupting the temperature
• Forced air circulation for uniform temperature distribution
• Corrosion-resistant stainless-steel interiors for long-term durability

Bacteriological incubators are one f the most crucial devices of modern laboratories, enabling vital research, diagnostics, and industrial applications. From isolating pathogens and developing life-saving drugs to ensuring the safety of food and water, these incubators are indispensable in maintaining the integrity and reliability of several processes. Is it time to boost the productivity of your laboratory? Discover high-performance bacteriological incubators and advanced lab equipment designed for reliability, accuracy, and compliance at IGene Labserve. Navigate here https://www.igenels.com/  to connect with our experts today.

Where Are Vertical Autoclaves Used?

 

Vertical autoclaves are the quiet workhorses of sterilization, making sure everything from surgical instruments to research media is germ‑free and ready to use. You’ll find them humming away in hospital CSSDs and OT rooms, decontaminating loads in microbiology labs, safeguarding patient care items in nursing homes, and supporting breakthrough experiments in research centers. Wherever absolute sterility is non‑negotiable, a vertical autoclave is close at hand, delivering fast, space‑saving, reliable performance.

How iGene is Transforming the Future of Molecular Diagnostics?

 

Molecular Diagnostics has revolutionized disease detection, monitoring, and treatment. As this sector is slated to grow further ahead with a good pace, companies offering reliable and cutting-edge laboratory solutions are one of the biggest contributors to the advancement of biomedical sciences. One company that is at the forefront of innovation and transformation is iGene Labserve Private Limited based in New Delhi. It has carved out a niche in the field of scientific equipment, especially in molecular biology and diagnostics.

Overview of iGene Labserve

iGene Labserve is one of the leading providers of laboratory instruments that cater to diverse fields like genomics, drug discovery, proteomics, biopharma, food safety, agriculture, and environmental sciences. Being committed to innovation and quality, the company supports researchers, laboratory professionals, and clinicians actively by delivering superior-quality tools that empower diagnostic and scientific workflows.

If you take a glimpse at the product portfolio of iGene Labserve, you will realise how the company has successfully addressed the challenges in real world with state-of-the-art lab devices. From gel documentation systems, PCR machines, to biosafety cabinets, and incubators, the list is endless. The company offers a wide range of tools that are designed to meet the requirements of molecular diagnostics lab.

Advanced tools for diagnostics

At the core of iGene Labserve’s success is an array of devices that play a vital role in advancing molecular diagnostics. Here they are:

·         PCR machines

PCR machines are widely used to quantify and amplify DNA and RNA sequences, which is essential for identifying bacteria, viruses, and genetic mutations. qPCR machines emerged as a cornerstone of diagnostic procedures during the global pandemic across the globe.

·         Gel documentation systems

Gel electrophoresis and documentation systems enable researchers to separate, visualize, and analyze RNA, DNA, and proteins with precision. These systems have created a breakthrough in forensic science, genetic research, and diagnosis for treating diseases through the interpretation of molecular changes at the cellular level.

·         ELISA reader

Another crucial tool that is used for immunological assays is ELISA reader that helps in accurate quantification of hormones, biomolecules, and antibodies, offering insights into the immune response of the patient, especially when dealing with cancer screening and diagnosis of infectious diseases.

Applications of iGene Labserve’s instruments in the diagnostic domain

The impact of the instruments of iGene Labserve Private Limited spans across several domains in molecular diagnostics.  Here are some notable applications to note:

·         Analysis of genetic mutation

The laboratory instruments of iGene help detect genetic mutations and variations that often expose people to diseases like cancer or inherited conditions that aid in personalized medicine.

·         Detection of infectious diseases

PCR machines are widely used for detecting viral and bacterial pathogens, enabling early diagnosis and improving patient management strategies.

·         Pharmacogenomics

Laboratories equipped with iGene’s devices can assess how genes may impact a person’s response to drugs; that way, doctors can choose the best treatments with minimal side-effects.

Commitment to innovation and quality

At the core of the philosophy of iGene Labserve is dedication to quality, innovation, and consistency. The company complies with strict manufacturing and quality control protocols that ensure the reliability, durability, and accuracy of devices. Each device here is designed, keeping in mind the end user, ease of use, and ease of maintenance. The company ensures that their offerings are good enough to the requirements of the scientific community.

Future outlook: Strengthening healthcare

The future of molecular diagnostics lies in personalized and predictive healthcare, and iGene Labserve is well-positioned to support this paradigm shift. As diagnostic labs look for more accurate, faster, and cost-effective methods, the demand for dependable instrumentation will continue to grow.

By continuing to expand their capabilities and developing smarter equipment, iGene Labserve is poised to play a key role in advancing how diseases are understood and treated. Their technology not only facilitates early and accurate diagnosis but also empowers researchers to make groundbreaking discoveries that could redefine patient care.

iGene Labserve Private Limited is more than just a laboratory equipment supplier ; it’s a driving force in the molecular diagnostics revolution. With a clear focus on quality, innovation, and end-user collaboration, the company is helping shape the future of diagnostics and life sciences in India and beyond. Are you ready to elevate your laboratory’s capabilities and stay ahead in the fast-evolving world of molecular diagnostics? Partner with iGene Labserve, India’s trusted provider of cutting-edge scientific instruments designed to deliver precision, reliability, and innovation. Click here https://www.igenels.com/ to empower your research right away.

The Benefits Of Freeze Dryer

 

Freeze drying, also known as lyophilization, is a powerful preservation method that removes moisture from food and other materials while maintaining their original flavor, texture, and nutrients. It extends shelf life, reduces weight for easy storage, and eliminates the need for preservatives, making it ideal for food storage, pharmaceuticals, and biotechnology applications

The Vital Role of Freeze Drying in Advancing Pharmaceutical Research

 

The pharmaceutical industry depends on the accuracy of information related to all those structures that are to be targeted for research. When studying an organism or sample that is to be prepared for analysis, retaining its integrity is one of the most vital steps. Moreover, the industry also requires drugs that are relatively easy in terms of storage and transportation and reduce the risk of degradation. One of the most suitable methods that play a crucial role in advancing pharmaceutical research and storage of drugs is freeze drying.

Understanding the freeze drying process

The freeze dryer equipment conducts the freezing and drying process in three different steps:

• Freezing

In this stage, the pharmaceutical product undergoes freezing only to convert water into ice.

• Sublimation

During sublimation, the ice is removed by converting solid ice into vapor under reduced pressure conditions.

• Desorption

In the final stage, traces of residual moisture undergo removal, thereby making the product stable and long-lasting.

By using the freeze dryer, you can make the final product porous that can be easily reconstituted with a solvent, usually water prior to use. This technology is especially useful for those drugs that are heat-sensitive and tend to degrade under the impact of conventional drying or other methods of storage.

Significance of freeze drying for pharmaceutical research

Here are the reasons why freeze drying is important for pharmaceutical research:

1. Increases stability of vaccines and biologics

Several of the modern-day drugs are made using complex molecules that are derived from living organisms like antibodies, nucleic acids, and proteins. Now, these molecules are extremely sensitive to humidity, temperature, as well as light, making it challenging for researchers to preserve the products.

Fortunately, the freeze drying techniques helps stabilize these substances without compromising their structural integrity. The freeze dryer also assists in the preservation of vaccines at ambient temperatures inside the refrigerator, making them more accessible in low-resource settings.

2. Retaining the efficacy and potency of drugs

If you have been researching in the pharmaceutical laboratory, you must be familiar that several of the active pharmaceutical ingredients or APIs tend to lose their potency and efficacy when the moisture and temperature levels are high. Freeze drying helps preserve the therapeutic content of the sensitive compounds like enzymes, peptides, and the monoclonal antibodies. That way, administering effective treatment becomes easy even when the drugs remain in the storage for longer hours.

3. Increases shelf-life of drugs

One of the most noted benefits of using a freeze dryer in the pharmaceutical laboratory is boosting the shelf life of various drugs. The absence of water prevents degradation reactions like hydrolysis and oxidation. Due to this, the freeze-dried pharmaceutical products remain intact and stable for many years when stored properly. That way the efficiency of pharmaceutical supply chains improve radically.

4. Aids delivery and reconstitution of drugs

Freeze-dried products are compact and lightweight. Therefore, it helps prevent the hassles of transportation and storage. Moreover, the reconstitution is easy and simple during the time of administering the drugs. This technique is especially useful for handling medical situations in areas where healthcare infrastructure is limited.

Applications of freeze drying process in drug development

What are the applications of freeze drying process in drug development? Here is what you need to know:

• Pace up research timelines

During the initial stage of drug discovery, researchers tend to work with extremely little quantities of valuable compounds that are also rare. With freeze drying, sample preparation becomes safe and effective, reducing the chances of loss and degradation. It helps in expediting pre-clinical studies and helps in spotting candidates for trial.

• Improve drug stability

The freeze dryer equipment allows researchers to experiment with multiple formulations, thereby enhancing stability and delivery. Researchers can optimize characteristics of various products like the rate of dissolution, solubility, and mechanical integrity by understanding the behavior of products during the freeze-drying cycle.

• Assist in clinical trials

During the procedure of clinical trials ensuring the stability of drugs that are to undergo investigation is important. With freeze-dried formulations, you will come across a reliable way to ensure consistency in the trial sites.

With continued efforts of innovation, the demand for freeze dryer is likely to grow. It is not just another technique of preservation but a milestone technique that is going to enable pharmaceutical research and innovation. As research procedures push ahead of restrictions, freeze-drying or lypholization is one of the trustworthy procedures that will expectedly enhance good health.

A Comparison between High-Speed and Ultrasonic Homogenizers for Tissue Sample Preparation

 

For tissue homogenisation, laboratory professionals and researchers often face the dilemma when selecting a suitable tissue homogenizer to meet their needs. Typically, there are two types of homogenizers – high-speed and ultrasonic. Although both devices aim to achieve the same goals, which is to break down tissue samples, each one has its set of pros and cons. To streamline the decision, you need to analyse the features, advantages, and disadvantages of both the devices to make the right decision but first let us understand what tissue homogenization is.

Things to know about tissue homogenisation

Tissue homogenisation is a process where tissue samples undergo disruption to extract nucleic acids, proteins, lipids, and various cellular components. The primary reason for researchers to conduct this process in the laboratory is to break the cells without damaging the parts that are to be used for further analysis. Now, what’s really important is the method of homogenisation as it may impact both the quantity and quality of various extracted materials.

There are two ways to achieve homogenisation – using high-speed and ultrasonic devices. Here is a detailed account of high-speed vs ultrasonic homogenizer for you to choose the right equipment.

High-speed tissue homogenizer

A high speed tissue homogenizer comprises a motorized system operating at very high speed that is enough to shear the tissues. In this device, the movement of the rotating blades help in breaking down tissues into very small particles.

Usually, the sample tissue is placed in a homogenisation tube and the rotor blades are submerged into the samples. When the rotor spins very fast, it creates an amount of force that helps in breaking down the tissues and the release of intracellular components.

Pros of high-speed tissue homogenizer

Wondering what makes the high-speed homogenizer a suitable device for studying the intracellular components?  Here is what you need to know:

• Efficiency and speed

A high speed tissue homogenizer can break down volumes of samples in one go and this is particular useful for laboratories where homogenisation of multiple tissue samples need to take place within a short time.

• Scalability

This type of homogenizer is available in a wide range of sizes which makes it easy to choose one that suits your workspace.  So, you can get small handheld units or larger models for small and large volume applications.

• Versatile device

The high-speed device is capable of handling various types of tissues, whether soft or hard like liver and muscles without requiring specialised attachments.

• Consistency

Due to high shear force that the rotor generates, the homogenization achieved is consistent across multiple samples.

• Cost-effective

High speed homogenizers are usually more cost-effective compared to ultrasonic homogenizers when you compare its capacity of processing high-volume and multiple samples.

Cons of high-speed homogenizer

Here are a few disadvantages of high-speed tissue homogenizer:

• Generation of heat

Due to high-speed rotational motion, high-speed tissue homogenizers often generate very high amount of heat, causing degradation of heat-sensitive compounds when the setup is not controlled carefully.

• Damage to samples

While high-shear forces are useful for disrupting tissues, it may also lead to damage in the samples, especially for those intracellular components that are sensitive to mechanical stress.

• Limited control

While homogenizers are good for disruption of tissues, it lacks the fine control that is needed for those applications where precision matters like handling fragile or delicate samples.

Ultrasonic homogenizer

The ultrasonic homogenizer uses very high-frequency sound waves also called ultrasound to disrupt tissue samples. Here, repaid pressure changes take place in the liquid medium and create small bubbles that explode and create shear forces that leads to disruption of tissues.

You need to just place the samples in the liquid medium and a probe that emits ultrasound waves, which cause the tissues to disrupt and release of cellular components.

Pros of ultrasonic homogenizer

If you want to delve into the differences in homogenisation, here is a detailed of the advantages of using an ultrasonic homogenizer ad here they are:

• Reduced generation of heat

Although ultrasonic homogenizers generate a lot of heat, they can perform equally well in cooler environments and reduce the risk of thermal damage to a great extent. That is one of the reasons why they are preferred for heat-sensitive samples.

• Control and precision

Ultrasonic devices offer good control over the process of homogenisation and allow users to adjust various parameters like amplitude, power, and time to achieve the desired results. This is especially useful for those tissue samples that are highly delicate.

• Homogenisation in smaller volumes

With this device, processing small volumes of samples are good for different research applications. The ultrasonic homogenizers are highly useful for situations where researchers need to obtain high throughput with limited amount of samples.

• Good for tough materials

When it comes to disruption of tough tissue samples, ultrasonic homogenizers are highly effective. Samples comprising tough and fibrous tissues that are otherwise difficult to homogenize becomes easy with this device.

Cons of ultrasonic homogenizer

You need to get glimpses of the reasons why you might avoid using an ultrasonic homogenizer:

• Time and cost

Although ultrasonic homogenizers are deemed as appropriate for smaller samples, the processing time shoots for large volumes of samples.  Moreover, they are usually more expensive than high speed homogenizers.

• Scalability is limited

These devices are not useful for large-scale homogenization due to smaller probe size and restricted power of processing, making them largely suitable for smaller samples.

• Inconsistent results

The efficiency of homogenization using the ultrasonic device depends on various factors like volume and types of samples and the positioning of the probe. That way, the results you obtain may become inconsistent.

Both high-speed tissue homogenizers and ultrasonic homogenizers are indispensable tools for disruption of tissues. Each has its advantages and disadvantages in terms of speed, versatility, and efficiency and processing volumes. You need to identify your specific needs before making the decision. Here is how you need to identify your needs:

• For large volumes of tissue samples and multiple samples, a high-speed tissue homogenizer is a cost-effective option for laboratories
• For tough and fibrous tissues like seeds, bone, and plant materials, the ultrasonic homogenizer provides better results.
• For processing small volumes of samples one at a time, the ultrasonic homogenizer provides better control but it won’t be useful for multiple samples
• For laboratories running under shoestring budget, the high-speed tissue homogenizer is most versatile for generic tissue disruption.

At IGene Labserve, one of the renowned manufacturers and suppliers of high-grade laboratory devices, you just have to place your order right away. Navigate here https://www.igenels.com/ to check our lab equipment.

The Role of Precise Temperature Regulation in High-Speed Centrifugation

 

High speed centrifugation in one of the techniques used widely across molecular biology and biochemistry labs. Furthermore, it is also used for medical research. The device makes samples spin at very high speed for the components to separate based on their density, size, and shape. Although centrifugation is one of the powerful tools used for preparing samples, the process is equally sensitive to temperature fluctuations. Therefore, temperature control in high-speed cooling centrifuge is a matter of concern for researchers; the more precise the temperature better is the outcome.

What is high-speed centrifugation?

High speed centrifugation is the method of spinning samples at extremely high speed of over 10,000 RPM. The main goal of this equipment is separating different components of a sample like nucleic acids, proteins, macromolecules, and organelles. Usually, the centrifugal force that the device generates during spinning makes the particles move to different layers within the sample, which is known as sedimentation. The technique in various applications like:

• Purification of proteins where specific proteins are isolated from within a mixture
• Extraction of RNA or DNA where nucleic acids need to separate from cellular debris
• Cell pellets that takes place in laboratories for analysis
  

Role of temperature in high-speed centrifuge

Temperature plays a vital role in high-speed centrifugation and impacts the efficiency of the separation process as well as the integrity of the samples.

Read here to understand the significance and role of temperature regulation in high-speed centrifugation devices:

1. Preventing degradation of samples

One of the primary issues that arise during high-speed centrifugation is the risk of sample degradation. When it comes to biological samples like enzymes, nucleic acids, and proteins, temperature-controlled centrifuge is a more realistic solution.

The reason is that it helps control the high heat that generates during the process and prevents the formation of unwanted thermal effects. Due to excessive heat, the function and structure of various biomolecules may be altered and proteins undergo denaturation, and enzymes lose their catalytic activity. At the same time, nucleic acids may be damaged due to oxidative degradation or hydrolysis. That is why having control over temperature is a must-have during the centrifugation process.

2. Optimising the efficiency of sedimentation

Uncontrolled temperature may affect the sedimentation process as well. During high-speed centrifugation, the particles separate based on their density and size. Now, the speed of movement of the particles depends not only on the force applied but the viscosity of the medium. As temperature impacts the viscosity of liquids, it tends to reduce when the temperature is high.

Due to reduced viscosity, the rate of sedimentation may change when working with complex or sensitive samples. With temperature control, viscosity becomes stable and the separation of components takes place appropriately. Eventually, the accuracy of the experiment improves to a great extent.

3. Minimises the build-up of heat

The process of spinning a sample in a cooling centrifuge is bound to generate very high heat due to fast movement. With increased speed of spinning, the heat production increases. Now, unless the heat dissipates faster, it may lead to an abnormal increase of sample temperature and create an effect of localized heat. Due to such fluctuations in temperature, the variability of the sedimentation process changes greatly and the results become inconsistent. Keeping the temperatures low prevents buildup of excessive heat and the reliability of the results improves greatly.

4. Boosting the recovery of samples

One of the objectives of using a high speed cooling centrifuge device is boosting the recovery of samples like cells, nucleic acids, and proteins. Without regulating the temperature, the nature of the sample may be compromised greatly and the yield goes down as well.

For instance, when the temperature builds up beyond limits, the fragile calls may be destroyed. That is why controlling the temperature in a high-speed cooling centrifuge is essential to recover the target components of the sample, resulting in good yield and desired results.

5. Improving reliability of the results

Centrifugation is a standardised process taking place in scientific research fields. However, temperature fluctuations often lead to inconsistencies between successive runs. For instance, when one run takes place at a specific temperature, which is higher than the other, the proteins might denature faster than expected, resulting in the variation of results.

So, you are familiar now that temperature control is an essential aspect of high-speed centrifugation. It is one of the most important considerations that one needs to consider as it impacts the reliability of the entire process. It is necessary to maintain low and constant temperatures throughout the process of centrifugation to ensure that the sensitivity of the samples remain intact. At IGene Labserve, one of the best-known suppliers of lab equipment, we sell top-grade equipment for laboratories. Navigate the website here https://www.igenels.com/  to learn more about the prices and the features of lab equipment.

What to Expect at a Pharmaceutical Equipment and Machinery Expo?

 

Pharmaceutical machinery expos are mainstream and crucial events bringing under one roof manufacturers, innovators, and professionals. They have merged as dynamic platforms for networking, sharing knowledge and showcasing innovative technology and solutions. Amptech India Pharma Machinery Expo 2025 is one of those events where seasoned laboratory tech manufacturers and suppliers will expectedly share the platform with newcomers to shape the world of innovation.

Here are the things one needs to expect during the machinery expo:

1. Showcasing the pharmaceutical machinery

The primary aspect of any pharmaceutical machinery expo is showcasing the latest and innovative machines that are slated to transform the industry. From incubators, shakers to water baths, fume hoods, laminar air flow bench and other equipment, the expos usually present the most advanced solutions that will expectedly improve the quality, efficiency, and other aspects in the pharmaceutical manufacturing industry. Here is the list of IGene Labserve products you are to come across in the Amptech Expo:

• Laboratory fume hoods
• Laminar air flow bench
• Cooling incubator shaker
• Digital water bath
• Biosafety cabinet class II
• Carbon-dioxide incubator
• Gel documentation system
• Freeze dryer
• High-speed refrigerated centrifuge
• Double beam spectrophotometer
• Rotary evaporator
• Vacuum controller

By participating in Amptech India Pharma Machinery Expo 2025, manufacturers can provide live demonstration of machines and offer insights into the benefits as well as the functionality. Moreover, the buyers get a chance to personally interact with equipment manufacturers and move forward their queries regarding the specifications and the capabilities of these machines.

2. Innovative solutions and cutting-edge technology

The pharmaceutical expos are a hub for discovering the latest advancements in the field of technology. As the industry is fast moving towards innovation and automation, the laboratory equipment manufacturers are also trying to catch up with the latest trends that are likely to shape the industry within the next few years. While the visitors get first-hand account of how the equipment works and also ask questions about the application of this device.

3. Industry trends and regulations

The pharmaceutical industry has witnessed rapid changes in the recent years and not just the products but the trends and regulations as well. No wonder, these expos serve as a wonderful place and allow the manufacturers and buyers to stay informed about the latest developments. So, the manufacturers and buyers may hear about the following:

• Market trends: The industry is not restricted today and has spread wings across the world. That way, none may learn about regulations and manufacturing of equipment in different regions. Also, companies expand their knowledge about domestic as well as international growth.
• Regulatory changes: The regulatory bodies that control the standard of manufacturing goods in the pharmaceutical industry update their standards.

 

4. Workshops and sessions

The Amptech India Pharma Machinery Expo 2025 will also have workshops and educational sessions on the latest trends. Such industry-led sessions are held by experts and cover the recent changes and trends in the machinery as well as the best practices to follow for safety and optimisation.

Overall, the Amptech India Pharma Machinery Expo 2025 that is to be held in Sidcul, Hardwar, Uttarakhand is going to be home for the best-known and reliable equipment manufacturers. Attending this event is going to open the eyes of participants to networking, learning about the latest technologies, and seeking opportunities of partnerships.

From live demonstrations to workshop and sessions, the companies are gearing to showcase their areas of expertise for the upcoming expo. If you are a participant, expect to gain invaluable experience to propel your business forward. IGene Labserve is one of the participants of this expo and will expectedly showcase various products that may revolutionise their business and the industry as a whole during the coming years.

How to Prolong the Life of High-Speed Tissue Homogenizer with Effective Maintenance?

 

High speed tissue homogenizers are extensively used across biological, biochemical, and pharmaceutical industries. They are primarily used to break down various cells and tissues into smaller size to carry out further analysis as well as helps in protein extraction and DNA or RNA analysis. So, it’s clear that maintaining the high speed tissue homogenizer is vital to ensure consistency of performance, extension of its lifespan, and minimizes the downtime. That is why we will explore here a few maintenance tips that may help increase the life of a homogenizer.

Listed below are some vital maintenance tips of a high speed tissue homogenizer that helps prolong its life:

1. Scheduling inspections

Scheduled inspection is pivotal to ensure that the laboratory tissue homogenizer stays in good shape. You can schedule weekly, monthly, or yearly maintenance based on the condition of the equipment and its functionality. With these inspections, you will find it easier to spot the issues and conduct maintenance tasks to make sure that the homogenizer delivers the desired outcome. Usually high-speed tissue homogenizers require inspection more frequently compared to those that function under low-pressure.

2. Storing the homogenizer

It is not just about maintaining the equipment but storing it properly makes a difference as well.  So, what are the storing techniques of the equipment you need to follow?  The first step is storing the homogenizer in cool and dry environment and far away from moist and humid places that may damage the motor coils and electrical components.

Furthermore, one needs to safeguard the device from contaminants and dust. The best solution is to cover the machine with a protective cover to prevent the dust from not entering the motor or probes. Finally, the components of the device like the adapters, additional rotors, and probes stay inside dedicated storage cases. Not handing the smaller parts of the device properly may lead to incessant damage.

3. Cleaning and sanitisation of the equipment

Just like other laboratory devices, a high-speed tissue homogenizer needed in the field of biomedical research must undergo regular cleaning. Naturally, after using the device each time, cleaning ensures that you can eliminate the chances of contamination, maintain the functionality of the motor as well as the other components. Although special cleaners may be needed for removing the tissue or protein buildup, you must avoid using harsh chemicals for cleaning may degrade the quality of parts, especially the metal-made components. After cleaning, the components must be made dry to prevent corrosion and rusting.

4. Maintaining and lubricating the moving parts

Some of these devices contain moving parts like shafts and motors which when not lubricated properly might result in wear and tear. However, do not over-lubricate the device as it may attract more contaminants and dust, resulting in corrosion and clogging. So, try to apply only a small amount of lubricant and check the parts for overheating. Overheating in a high speed tissue homogenizer may lead to reduced performance or failure of motor.

5. Maintaining the motor and electrical system

Are you aware that the motor is the heart and soul of the homogenizer? If you aren’t, you need to conduct proper maintenance of these systems for the equipment to function properly. The motor also attracts debris and dust that often accumulates in the housing, which may result in short circuit and overheating. So, the housing must stay clean.

Besides, the electrical cables need to be examined for signs of corrosion, wear and tear as well as fraying. So, the moment you notice any damage, you must replace them as soon as possible to keep the chances of faults at bay. At the end, check the power supply thoroughly to ensure that it is stable and consistent. Power interruptions or surges may result in permanent damage.

6. Follow the specifications during usage

High speed tissue homogenizers are used for specific purposes. However, researchers often mishandle this device or rely on them beyond the recommended usage. That is why you must never overload the machine with enormous samples as it will strain the motor and lower the lifespan of the device. Furthermore, the tissues must undergo adequate preparation based on the guidelines of the manufacturer for proper homogenization.

So, you are familiar with all those maintenance tips that are going to play a key role in extending the life of your laboratory tissue homogenizer. You need to simply dedicate your time and effort to keep the device functioning properly for years. At IGene Labserve, we have mastered the art of manufacturing top-grade laboratory equipment. So, just take a look here https://www.igenels.com/ and order right away.

Real Time PCR Vs Traditional PCR: A Guide to Choosing the Right One for Your Lab

 

Polymerase chain reaction or PCR is a technique used in molecular biology to analyse and enlarge RNA and DNA samples. It is widely used for conducting research, maintaining quality control, and in the field of diagnostics. However, when buying a PCR machine, you may feel confused when choosing between traditional or real-time machine. There are differences between traditional or endpoint PCR and real-time or quantitative PCR machine. You need to know the differences between the devices to understand which one is the right fit for your laboratory.

What is a traditional PCR machine?

Traditional PCR is a well-established and familiar technique that has been used in laboratories for decades. It helps augment the RNA and DNA sequence through repetition of cooling and heating cycles. That way, the DNA strands become separated and aid in DNA replication. Once the cycles are completed, the visualisation process takes place through gel electrophoresis.

The products that have been amplified are then stained with a dye; thereafter the intensity of bands on the gel shows the quantity of enlarged DNA. Although the traditional PCR machine performs reliably and has been used in research, forensic science, and diagnostics for a long time, it fails to meet the gaps when it comes to quantitative analysis and real-time data acquisition. So, when buying the best PCR machine for labs, you need to analyse the features and specifications of both to make an informed decision.

What is a real-time PCR?

A real-time PCR machine also called quantitative PCR scores over the traditional machine in several different ways. When using this machine, you need not wait until the completion of PCR to analyse and amplify DNA but come to know about the quantification when the DNA is amplified. This is possible through the monitoring of fluorescence that emits during the amplification process and provides continuous data in real-time. The fluorescence generates due to specific dyes that usually bind to the target DNA. Now, when the reaction progresses further, the fluorescence emitted binds with the quantity of DNA that undergoes amplification and eliminate the need to wait for the completion of PCR.

Undoubtedly, real-time PCR is a highly versatile method and extensively used for gene expression analysis, detection of mutation, viral load, and more.  The ability of this machine to measure DNA and RNA in real-time makes it a valuable tool for clinicians and researchers.

Understanding the key differences between traditional and real-time PCR

Here are the differences between real-time and traditional PCR machine:

• Quantification

1. The traditional PCR is limited when providing qualitative data and helps you identify whether a particular DNA sequence is present or absent in the sample. However, it cannot tell the amount of DNA in real-time and you need to wait till the completion of PCR analysis. Naturally, the quantification of DNA is not precise.
2. Real time PCR helps in precise quantification of data. The fluorescence that emits during the process of amplification allows researchers measure the amount of RNA and DNA within the samples at each cycle.

 

• Detection method

1. The traditional PCR is a highly sensitive machine and can detect even the lowest amount of DNA but it is restrained by post-PCR analysis. Naturally, it cannot detect low quantity of the target DNA.
2. Real-time PCR is relatively more sensitive compared to the traditional PCR. As DNA amplification is constantly monitored, DNA and RNA can be detected early in low-quantity samples.

 

• Effective timing

1. Traditional PCR takes a few hours to complete and you need to wait until post-PCR analysis to visualise the results. Naturally, you need more time to complete the entire experiment.
2. Real-time PCR delivers quick results as the quantification and amplification process takes place simultaneously. So, there is no need for you to wait until post PCR analysis. Naturally, the time taken is less, especially for high-throughput applications.

 

• Cost

1. The traditional PCR machines are more affordable compared to real-time PCR systems. However, you need to spend additionally for consumables, gel electrophoresis reagents like agarose gel and DNA staining agents. Therefore, the traditional PCR machines may add to the cost due to these requirements.
2. Real-time PCR machines are more expensive than traditional machines in terms of investment and maintenance but the accuracy of data justifies the cost to a great extent. So, if you analyse the real-time PCR machine benefits that way, making an informed decision is easier.

When choosing between traditional and real-time PCR machines, a lot depends on the specific requirements of the laboratory. So, if you are working with samples with low RNA and DNA abundance, real-time PCR machine is the right choice. However, if you are conducting basic DNA analysis where the most important thing is the absence and presence of a specific DNA sequence, the traditional PCR machine is more important. At IGene Labserve, we sell top-notch quality of laboratory equipment. Just explore the models here https://www.igenels.com/ and order the equipment you need right away.